摘要
目的探讨木脂素对醋酸铅染毒小鼠生精功能的影响及其作用机制。方法将昆明种雄性小鼠60只随机分为对照组、模型组、阳性对照组、木脂素组,除对照组外,其余3组小鼠均腹腔注射醋酸铅(40 mg/kg),连续10 d,建立雄性小鼠生精障碍模型;造模后第2 d开始,阳性对照组小鼠腹腔注射绒毛膜促性腺激素(500 IU/kg),木脂素组小鼠给予木脂素(200 mg/kg)灌胃,对照组及模型组小鼠灌胃等量生理盐水,连续30 d;末次给药24h后,处死小鼠,取睾丸和附睾,检测睾丸组织超氧化物歧化酶(SOD)、乳酸脱氢酶(LDH)、三磷酸腺苷(ATP)酶含量。比较各组小鼠精子存活率。苏木精-伊红染色观察睾丸组织形态。结果与对照组比较,模型组小鼠精子活率[(53.2±1.3)%]降低,LDH、SOD、Mg2+、Ca2+-ATP酶活性[分别为(0.18±0.03)U/gprot、(16.80±2.23)U/mgprot、(31.03±4.87)、(8.12±1.24)μmol Pi/(mgprot·hour)]降低,差异有统计学意义(P<0.01);与模型组比较,木脂素组小鼠精子活率[(68.7±2.2)%]升高;LDH、SOD、Mg2+、Ca2+-ATP酶活性[分别为(0.23±0.09)U/gprot、(23.01±3.86)U/mgprot、(64.21±10.49)、(17.20±5.18)μmol Pi/(mgprot·hour)]升高,差异有统计学意义(P<0.05)。结论木脂素对醋酸铅所致生精障碍小鼠的生殖功能具有一定保护作用,其机制可能与抗氧化损伤有关。
Objective To study effects of lignan on spermatogenesis and its mechanism in mice with lead acetate exposure. Methods Sixty Kunming male mice were randomly divided into a control group,model group,positive drug group,and lignans group. Except for those in the control group,all the mice were injected lead acetate for 10 days at the dosage of 40 mg·kg- 1·d- 1to establish spermatogenesis disorder model; two days after lead acetate injection,the mice in positive drug group were treated with human chorionic gonadotropin（ 500 IU·kg- 1·d- 1） via intraperitoneal injection and those in lignans group were given lignans（ 200 mg·kg- 1·d- 1） via gavage; the mice in the control group and model group were given normal saline; the experiment lasted for 30 days. Twenty-four hours after the last treatment,all the mice were sacrificed and testis and epididymis of the mice were sampled. Superoxide dismutase（ SOD）,lactate dehydrogenase（ LDH）,Mg2 ＋ATP enzyme and Ca2 ＋ATP enzyme in testicular tissue were detected and sperm viability was compared between the groups and the morphology of testicular tissue were observed with hematoxylin-eosin staining. Results Compared with the control group,sperm motility was reduced; SOD,Mg2 ＋ATPase and Ca2 ＋ATPase contents were decreased; and LDH level was increased for the mice of the model group（ P〈0. 05,P〈0. 01）. Compared with the model group,sperm motility was increased,the level of SOD was increased,and that of LDH was decreased in the mice of lignans group（ P〈0. 05,P〈0. 01）. Conclusion Lignans could protect spermatogenesis from lead acetate-induced damages in mice and the mechanism of the effect may related to anti-oxidativation of lignans.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2015年第3期321-323,共3页
Chinese Journal of Public Health
基金
国家自然科学基金(81201671)
吉林省教育厅基金(2010254
2013359)
关键词
木脂素
醋酸铅
抗氧化损伤
生精功能
lignans
lead acetate
oxidative damage
spermatogenesis
作者简介
沈楠（1972-），女，吉林吉林人，副教授，硕士，研究方向：中药药理毒理。
通讯作者：任旷，E-mail：renkuang@sina.com.
