摘要
目的探讨蝙蝠葛碱对阿尔茨海默病(AD)转基因细胞模型氧化应激损伤的保护作用。方法将质粒APPswe转染入SH-SY5Y细胞中,构建过表达APP的AD细胞模型,检测不同蝙蝠葛碱浓度下细胞存活率、丙二醛(MDA)含量及COX-2蛋白表达的差异。结果随着给药浓度的增加,细胞存活率逐步增加,MDA含量逐步降低,其中空白对照组、20或10倍稀释蝙蝠葛碱预处理组与模型组差异有统计学意义(P<0.05或P<0.01),10倍稀释蝙蝠葛碱预处理组与100、50倍稀释蝙蝠葛碱预处理组差异有统计学意义(P<0.05或P<0.01);COX-2蛋白表达量随着给药浓度增加而逐渐下降。结论蝙蝠葛碱对AD转基因细胞模型的氧化应激损伤具有一定的抑制作用,且呈现出剂量效应。
Objective To explore the protective function of dauricine on oxidative stress inju- ry induced by APP over-expressing in Alzheimer＇s disease （AD） cell model. Methods APPswe plas- mid was transfeeted into SH-SYSY cells in order to build APP over-expressing AD cell model. Differ- ences of cell survival rate, malondialdehyde （MDA） and COX-2 protein expression with different con- centrations of daurieine were analyzed. Results The survival rate of cell increased and MDA content decreased gradually with the increase of medication concentration, and there were significant differ- ences between the model group and control group, 20 or 10-fold dilution daurieine pretreated group （P 〈0.05 or P 〈0.01 ）. There were significant differences between 10-fold dilution daurieine pretreat- ment group and 100-fold or 50-fold dilution daurieine pretreatment group （P 〈 0.05 or P 〈 0.01 ）. COX-2 protein expression decreased gradually with increasing of medication concentration. Conclusion Dauricine shows a dose effect of protection on oxidative stress induced by APP over-expressing in AD gene transfected cell model.
出处
《实用临床医药杂志》
CAS
2015年第1期7-9,16,共4页
Journal of Clinical Medicine in Practice
基金
广西壮族自治区卫生厅中医药科技专项课题(GZPT1248)
中国高校医学期刊临床专项资金(11321935)
作者简介
通信作者：陈薇，E-mail：709426883@qq．com
