摘要
目的:观察知母皂苷AⅢ对黑色素瘤B16和A375细胞生长及活化巨噬细胞亚硝酸盐的影响。方法:体外培养B16和A375细胞,知母皂苷AⅢ作用48,72 h,四唑盐(MTT)比色法检测其对细胞生长的影响;倒置显微镜观察其对细胞形态的影响;以脂多糖和干扰素γ诱导小鼠巨噬细胞活化,知母皂苷AⅢ作用24h后检测细胞上清液中亚硝酸盐含量。结果:16μmol·L-1知母皂苷AⅢ作用于B16细胞48,72h明显抑制细胞生长;4,8,16μmol·L-1知母皂苷AⅢ作用于A375细胞48,72 h明显抑制细胞生长。显微形态显示16μmol·L-1知母皂苷AⅢ作用B16细胞48,72 h细胞体积缩小,细胞出现空泡,细胞坏死,其余各剂量形态变化不显著;16μmol·L-1知母皂苷AⅢ作用A375细胞48,72 h细胞体积缩小,细胞出现空泡,细胞坏死,从4μmol·L-1知母皂苷AⅢ开始A375细胞出现大量空泡。与LPS+IFN-γ刺激RAW264.7细胞组比较,10μmol·L-1知母皂苷AⅢ作用24h后明显抑制亚硝酸盐含量(P<0.01)。结论:知母皂苷AⅢ能明显抑制黑色素瘤B16和A375细胞生长,并能抑制巨噬细胞炎症,其具有抗肿瘤作用和抗炎作用,其抗肿瘤作用可能与抑制肿瘤炎症有关。
Objective： To observe the effect of timosaponin AⅢ on the proliferation of cultured melanoma B16 and A375 cells and nitrite produced from macrophage activation. Methods： MTT assay was adopted to detect the cell growth inhibition. The morphological changes of B16 and A375 cells were observed under an inverted microscope. Nitrite production of activated mouse macrophages induced by lipopolysaccharide（ LPS） plus IFN-γ was measured by Griess assay. Results： Timosaponin A Ⅲ could significantly inhibit the growth of B16 cells at the concentration of 16 μmol·L- 1after the 48- and 72-hour treatment,and significantly inhibit the growth of A375 cells at the concentration ranged from 4 to 16 μmol·L- 1after the 48- and 72-hour treatment. Shrinkage,vacuoles and necrosis of B16 and A375 cells were observed after the 48- and 72-hour treatment by 16 μmol·L- 1timosaponin AⅢ,the other concentration of timosaponin AⅢ showed no notable effect on B16 cells and vacuoles of A375 cells appeared at the concentration from 4 to 16 μmol·L- 1. Compared with RAW 264. 7 stimulated LPS plus IFN-γ,timosaponin A Ⅲ could significant inhibit nitrite production of macrophage inflammatory cell model at the concentration of 10 μmol·L- 1（ P 0. 01）. Conclusion： Timosaponin AⅢ can inhibit the proliferation of melanoma B16 and A375 cells and macrophage inflammation,suggesting it has anti-tumor and anti-inflammatory effects. The anti-tumor effect of timosaponin AⅢ may be related to the inhibition of tumor inflammation.
出处
《中国药师》
CAS
2015年第2期181-185,共5页
China Pharmacist
基金
上海市皮肤病医院优青项目(编号:2013)
作者简介
通讯作者：潘会君Tel：18017336879E—mail：pppphhhhjjjj@163．com
