摘要
目的调查宁夏西吉县啮齿动物种群空间分布格局与自然疫源性疾病传播风险因素．为自然疫源性疾病的防控提供指导。方法2012、2013年，按照不同生境及海拔高度分层抽样的方法，在宁夏西吉县设立乡镇调查点，在每个调查点，采用鼠夹法，捕获啮齿动物，计算捕获率及啮齿动物的种群空间分布。采用免疫荧光法检测捕获鼠肺出血热病毒抗原，用双抗原夹心酶联免疫吸附试验（ELISA）检测捕获鼠血清出血热抗体：剖检鼠类，取肝脾组织压印后直接培养进行鼠疫病原学检测；用间接血凝实验进行鼠疫F1抗体血清学检测。结果共发现啮齿动物14种，隶属2目7科9属，黄鼠和姬鼠为优势种群，其中黄鼠占种群的49．56％（112／226），姬鼠占种群的34．51％（78／226）。野鼠型出血热、动物鼠疫病原学检验结果和血清学检测结果均为阴性。结论西吉县生态环境的改善，导致鼠类种群空间分布格局发生改变：动物鼠疫流行风险在减弱．而存在姬鼠型出血热的发病风险因素。
Objective To analyze the spatial distribution patterns of rodent community and the risk factors of disease transmission in natural loci of Xiji County; Ningxia, in order to provide a scientific basis for disease control and prevention. Methods Rodents were captured at different habitats in different latitudes which were selected with a stratified sampling method in 2012 - 2013. Capture rate of different rodent species and spatial distribution patterns of the animal community were analyzed. Antigen and antibody of hantavirus were detected in lung tissue and blood of the rodents with immunofluorescence and enzyme-linked immunosorbent assay （ELISA）, respectively. Serum F1 antibody of Yersinia pestis （Y. pestis） was detected with indirect hemagglutination test and Y. pestis from liver and spleen tissue was cultured in vitro. Results Fourteen species of rodents were captured, belonging to 2 orders, 7 families and 9 genera. Among them, ground squirrels and wood mouse were dominant species, accounted for 49.56% （112/226） and 34.51% （78/226） of the community, respectively. Infections of hantavirus and Y. pestis were not found in the rodent＇s community. Conclusion With the improvement of ecological environment in Xiji County, the spatial distribution patterns of rodent community is changing; the risk of zoonotic plague is reduced, but the risk of hemorrhagic fever with renal syndrome is being.
出处
《中华地方病学杂志》
CAS
CSCD
北大核心
2015年第2期136-138,共3页
Chinese Journal of Endemiology
关键词
啮齿目
鼠疫
肾综合征出血热
Rodentia
Plague
Hemorrhagic fever with renal syndrome
作者简介
通信作者：乔富贵，Email：melody68man@163．com.
