摘要
目的 探讨添加丙氨酰谷氨酰胺的血液保存液与ACD配方保存液对储存血液红细胞膜Na＋K＋-ATP、Ca2＋Mg2＋-ATP酶活性的影响。方法 选取血标本分实验组A和对照组B,每组均为相对应的1~10管;A组保存液为ACD配方保存液和丙氨酰谷氨酰胺注射液,B组保存液为ACD配方保存液,比较两组采血后第1、3天红细胞膜Na＋K＋-ATP、Ca2＋Mg2＋-ATP酶活性,并进行统计分析。结果 A、B两组标本第1、3天分别测定红细胞膜Na＋K＋-ATP及Ca2＋Mg2＋-ATP酶活性,其中A组Na＋K＋-ATP酶活性分别为16.51±5.23、11.60±5.11,B组为12.90±6.01、6.31±3.67,A组Ca2＋Mg2＋-ATP酶活性分别为16.09±6.03、11.75±6.70,B组为12.38±5.37、5.05±3.23,A与B两组组间及组内比较差异均存在统计学意义（P〈0.05）。结论 ACD配方血液保存液加入丙氨酰谷氨酰胺制成血液保存液,与ACD配方血液保存液对存储血液红细胞膜Na＋K＋-ATP、Ca2＋Mg2＋-ATP酶活性影响的差异有统计学意义,对红细胞膜保护作用优于ACD配方保存液。
Objective To explore the influence of stored blood erythrocyte membrane Na＋ K＋ATP, Caz＋, Mgz＋ ATPase activity using the new blood preservation liquids of alanylglutamine and ACD formula. Methods To allocate the blood specimen to the experimental group A,and group B as control ,with 1-10 tubes in each group. To group A the ACD formula and alanylglutamine were added, and only ACD formula was added to group B. The values of Na＋- and K＋- ATPase and Ca2＋- and Mg2＋ -ATPase activity on the erythroeyte membrane were compared on days 1 and 3 after blood collection. Results The values of Na＋ and K＋ ATPase activity were 16.51±5.23 and 11.60±5.11 in group A, and 12.90±6.01 and 6.31±3.67 in group B. Ca2＋- and Mg2＋-ATPase activity were 16.09±6.03 and 11.75±6.70 in group A,and 12.38±5.37 and 5.05!3.23 in group B. All values of the ATPase activity showed significant difference between groups A and B （P〈0.05）. Conclusion Combination of ACD formula with alanylglutamine has better effect on the preservation of red blood cells than the ACD formula alone.
出处
《临床输血与检验》
CAS
2015年第1期49-51,共3页
Journal of Clinical Transfusion and Laboratory Medicine
关键词
血液保存液
抗凝剂
红细胞膜
ATP酶活性
Blood preservation Anticoagulant Erythrocyte membrane ATPase activity
作者简介
李大鹏（1964-），男，辽宁锦州人，副主任技师，学士，主要从事临床输血研究（Tel）0538—8839734，13583848186（Email）lidapeng1964@126．com。
