摘要
目的比较宫颈上皮内瘤病变(cervical intraepithelial neaplasia,CIN)组织标本中人乳头瘤病毒(human papillomavirus,HPV)感染的基因型分布情况及其临床意义。方法采用聚合酶链式反应和基因芯片检测技术对53例CINⅠ、67例CINⅡ和188例CINⅢ病变组织标本进行23种HPV基因分型检测,并对受检者进行相关资料分析。结果 53例CINⅠ患者检出HPV感染者29例,总的HPV感染率为54.72%(29/53);67例CINⅡ患者检出HPV感染者51例,总的HPV感染率为76.12%(51/67);188例CINⅢ患者检出HPV感染者175例,总的HPV感染率为93.09%(175/188)。结论 CINⅠ、CINⅡ和CINⅢ组织中总的HPV感染率存在着明显的差异性,聚合酶链式反应与基因芯片检测技术可应用于宫颈组织标本检测,一次可检测23种HPV基因型别,对我国女性宫颈HPV感染的分子流行病学调查、宫颈癌瘤的防治及其疫苗研究具有非常重要的意义。
Objective To compare the distribution situation of human papillomavirus（HPV）genotypes in the tissue specimens of cervical intraepithelial neoplasia（CINⅠ,CINⅡ and CINⅢ）and its clinical significance.Methods The polymerase chain reaction （PCR）and gene-chips technology were utilized for detecting 23 kinds of HPV genotypes in the cervical tissue specimens from 53 ca-ses of CINⅠ,67 cases of CINⅡ and 188 cases of CINⅢ.And the related data of all subjects were analyzed.Results Among 53 ca-ses of CINⅠ,29 cases of HPV infection were detected with the total HPV infection rate of 54.72%9;among 67 cases of CINⅡ,52 cases of HPV infection were detected with the total HPV infection rate of 76.12%;among 188 cases of CINⅢ,175 cases of HPV infection were detected with the total HPV infection rate of 93.09%.Conclusion The significant difference of the total HPV infec-tion rate exists in the tissues of CINⅠ,CINⅡ and CINⅢ.PCR and the gene-chip technology can be applied in the detection of the cervical tissue specimens,23 kinds of genotypes can be detected by once detection,which has very important significance to the mo-lecular epidemiological survey of female cervical HPV infection,prevention and treatment of cervical cancer and the HPV vaccine re-search.
出处
《国际检验医学杂志》
CAS
2014年第23期3180-3182,共3页
International Journal of Laboratory Medicine
关键词
宫颈
组织
上皮内瘤变
人乳头瘤病毒
基因型
cervix tissue intraepithelial neoplasia human papillomavirus genotyping
作者简介
朱晓珏，女，副主任技师，主要从事临床医学检验研究。
通讯作者，E—mail：29317167@qq．com
