摘要
研究了不同提取剂和提取条件对红曲霉玉米粉豆渣发酵液中Monacolin K的超声提取效果。实验以双波长紫外分光光度法检测发酵液中的Monacolin K浓度为指标,在单因素实验的基础上,通过正交实验及分析,确定超声提取工艺条件。实验结果表明:最佳提取剂为75%的乙醇,提取时间40 min,提取温度60℃,料水比(菌丝湿重∶75%乙醇)为1∶8(g∶mL),超声功率250 W。在此条件下,Monacolin K的提取率达到93.6μg/mL。
The effect of extractants and extraction conditions on the ultrasonic extraction rates of Monacolin K in fermentation broth of Monascus were studied. The optimal conditions were determined by orthogonal test and the Monacolin K in fermentation broth detected by dual wavelength ultraviolet spectrophotometry was chosen as the estimated index. The results indicated that the best extractant was ethanol and the optimal conditions were as follows： ultrasonic power was 250W, material liquid ratio （mycelial wet weight/g： 75% ethanol/mL） was 1 ： 8, extracting time was 40min,extracting temperature was 60℃ and ethanol concentration was 75%. Under these conditions, the extraction ratio of Monacolin K was 93.6 μg/mL. The extraction process is a viable method for extracting Monacolin K from fermentation broth of Mor^ascus.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2014年第4期216-220,共5页
Food and Fermentation Industries
基金
安徽省高校自然科学基金资助项目(kj2009a034)
芜湖市重点科技项目(芜科计字[2009]190号)
作者简介
学士，教授（通讯作者）。
