摘要
本研究采用农杆菌介导法(ATMT)将绿色荧光蛋白基因(gfp)转入轮枝镰孢菌(Fusarium verticillioides)获得了表达gfp的转化子。结果表明,转化子经过5代培养仍能发出稳定的荧光,转化后的菌落形态与菌丝生长速度差异不明显,对寄主仍有致病能力。通过PCR验证也表明gfp基因已成功转入到菌株PX1015基因组中,轮枝镰孢菌绿色荧光蛋白基因转化体的成功构建为该病菌侵染机制的研究奠定了基础。
In this study,transformants of expressing green fluorescent protein was obtained by delivering gfp gene into Fusarium verticillioides via agrobacterium tumefaciens-mediated transformation（ ATMT）. The result showed that the transformants had genetic stability and excellent fluorescence after 5 generations,and there were no significant differences in their colony morphology and mycelium growth rate,whose pathogenicity of host infection remained. PCR detection also indicated that the gfp gene had been transferred to the strain F. verticillioides PX1015. This study would provide a basis for the infection process using the green fluorescent transformant of F. verticillioides.
出处
《西南农业学报》
CSCD
北大核心
2014年第6期2374-2376,共3页
Southwest China Journal of Agricultural Sciences
基金
四川省财政基因工程专项资金项目(2011JYGC06-021)
现代农业(玉米)产业技术体系项目(CARS-02-15)
关键词
农杆菌
轮枝镰孢菌
绿色荧光蛋白
遗传转化
Agrobacterium tumefaciens
Fusarium verticillioides
GFP
Genetic transformation
作者简介
张小飞（1982-），男，博士，从事玉米抗病性研究
