摘要
目的:探讨自体骨髓基质干细胞(MSCs)移植联合促红细胞生成素(EPO)对大鼠脊髓损伤(SCI)治疗的效果。方法:培养与纯化骨髓基质干细胞。80只SD大鼠制备脊髓损伤模型,随机分为4组:干细胞移植组、EPO组、联合组和对照组。采用BBB法进行运动能力评分和组织切片观察来评定修复情况。结果:三个实验组与对照组相比,差异具有统计学意义(P<0.05),三个实验组均可明显促进大鼠运动功能和损伤后组织结构的恢复,其中联合组效果更显著。结论:骨髓基质干细胞移植联合EPO具有促进大鼠脊髓损伤后神经功能的修复。
Objective：To evaluate the effects of transplantation of autogeneic mesenchymal stem cells（MSCs） with erythropoietin（ EPO） therapy after rats spinal cord injury（SCI）.Method：MSCs were cultured and purified in vitro.The rat model with spinal cord injury was prepared by modified Allen method.Eighty rats were put into four groups：stem cell transplation group, EPO treatment group, combination group and control group.The motor function was evaluated with BBB score and the neurological function recovery was observed by HE staining.Result：Statistical differences were showed in inter-group（P〈0.05）.Compared with control group,in practical group,the motor function and morphological recoverment was obviously in MSCs combined EPO group.Conclusion：The neurological function of rats with spinal cord can be obviously improved by autogeneic mesenchymal stem cells transplantation combined with EPO.
出处
《中国医学创新》
CAS
2014年第32期1-3,共3页
Medical Innovation of China
基金
黑龙江省卫生厅科研项目(2012-283)
黑龙江省教育厅科研项目(12531746)
牡丹江医学院科研项目(2011-09)
作者简介
通信作者：孙晓冬
