摘要
目的 观察肾移植患者与霉酚酸（MPA）作用位点相关的次黄嘌呤脱氢酶（IMPDH）基因多态性对急性排斥反应的影响.方法 对105例肾移植患者采用基因测序法进行IMPDH基因多态性检测,并随访统计上述患者移植后1年内急性排斥反应的发生率,按照IMPDH不同基因型分组,分析IMPDH基因多态性与移植后1年内急性排斥反应的相关性.结果 105例患者中,所测3个位点中IMPDH1 rs2278293 GG型35例（33.33％）,GA型49例（46.67％）,AA型21例（20.00％）;IMPDH1 rs2278294 GG型22例（20.95％）,GA型49例（46.67％）,AA型34例（32.38％）;IMPDH2 rs11706052 GA型14例（13.33％）,AA型91例（86.67％）.单因素分析显示肾移植后1年内IMPDH1 rs2278293、rs2278294位点GG型患者急性排斥反应的发生率明显高于GA＋AA型患者,差异有统计学意义（P＜0.05）.两位点联合分析显示GG-GG型患者移植后1年内急性排斥反应的发生率更高,与GA＋ AA型者比较差异有统计学意义（P＜0.05）.结论 携带IMPDH1rs2278293、rs2278294的GA或AA型患者移植后1年内急性排斥反应发生率更低,对霉酚酸酯的治疗效果更好.
Objective To investigate inosine monophosphate dehydrogenase （IMPDH） gene polymorphisms involved in mycophenolic acid （MPA） response and their association with acute rejection one year after renal transplantation.Methods We genotyped three single nucleotide polymorphisms （SNPs） in 105 Chinese Han renal transplant patients.Genotyping was performed using derect sequencing SNPs Genotyping Assays.The association of SNPs with 1-year post-transplantat acute rejection was analyzed.Results Of the 105 patients,the IMPDH1 rs2278293 genotype frequencies of GG,GA and AA were 33.33%,46.67% and 20.00% respectively.The IMPDH1 rs2278294 genotype frequencies of GG,GA and AA were 20.95%,46.67%,and 32.38% respectively.The IMPDH2 rs11706052 genotype frequencies of GA and AA were 13.33% and 86.67% respectively.The IMPDH1 rs2278293 and rs2278294 were associated with acute rejection over the first year post-transplant.Combined rs2278293 and rs2278294 genotype was associated with acute rejection one year after renal transplant.Conclusion IMPDH1 rs2278293,and rs2278294 SNPs were associated with a lower risk of acute rejection and may improve MPA treatment outcome.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2014年第12期2678-2681,共4页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目（81273266）
江苏省临床医学科技专项资助项目（BL2013013、BL2014038）
江苏省医学重点学科资助项目（XK201151）
江苏省医学创新团队与领军人才资助项目（LJ201138）
苏州市科技基础设施建设计划项目（SZS201001、SZS0702）
关键词
次黄嘌呤脱氢酶
基因多态性
霉酚酸
急性排斥反应
肾移植
Inosine monophosphate dehydrogenase
Gene polymorphism
Mycophenolic acid
Acute rejection
Renal transplantation
作者简介
通信作者：何军，Email：junhe1964@163．com.
通信作者：侯建全，Email：xfl92@163．com
