摘要
目的探讨补肾中药对卵巢早衰(POF)卵巢储备功能及免疫调节影响,并观察对患者症状的改善,为临床治疗POF提供临床依据。方法随机选择2012年1月—2013年6月POF患者89例,根据治疗方案将所有患者分为对照组与观察组,对照组43例,给予戊酸雌二醇1 mg/d,口服,1次/d,连服21 d,最后5 d口服安宫黄体酮10 mg/d,1次/d,3个月为1个疗程。观察组46例,应用补肾中药治疗。水煎,1剂/d,口服,2次/d,连续服用3周,停用1周后开始下个疗程,连续服用3个月。观察患者治疗前后子宫和卵巢状况,采用放射免疫分析法测定血清促卵泡激素(FSH)、促黄体生成素(LH)、雌二醇(E2)含量,流式细胞仪检测T淋巴细胞CD4+和CD8+亚群。结果观察组患者治疗后子宫内膜厚度结果为(0.51±0.10)cm,高于对照组治疗后的(0.44±0.07)cm,差异具有统计学意义(t=4.17,P<0.05)。观察组患者治疗后FSH和LH分别为(36.08±1.37)U/L和(34.63±2.05)U/L,均低于对照组的(44.12±1.05)U/L和(39.14±1.88)U/L(t=5.19,4.99,P<0.05)。观察组治疗后E2为(75.89±6.74)pmol/L,高于对照组的(67.45±6.28)pmol/L(t=5.38,P<0.05)。治疗前对照组和观察组患者T淋巴细胞总数分别为30.68±3.79和31.02±3.16,治疗后分别为34.03±4.17和37.86±4.38,差异均具有统计学意义(t=5.24,4.91,P<0.05),且观察组高于对照组(t=5.17,P<0.05),观察组患者治疗后CD4+为47.11±1.21,高于对照组的40.42±1.67(t=5.80,P<0.05)。观察组治疗后CD8+为31.06±1.34,低于对照组的36.19±1.73(t=4.98,P<0.05)。结论补肾中药可明显改善POF患者症状和卵巢储备功能,调节雌激素水平,提高其免疫机能。
Objective To explore the clinical effect of kidney tonifying herbs on premature ovarian failure（POF） of ovari- an reserve function and immune regulation and the influence on the patient＇ s symptoms improved, and to observe the patient＇ s symptom and provide the clinical basis for the clinical treatment of POF. Methods 89 patients with POF were selected randomly from January 2012 to June in 2013 ,who were divided into the control group and the observation group according to the treatment methods. 43 in the control group were treated with Estradiol valerate 1 mg/day, oral, 1 times/d, and served 21 days,the last 5 days with oral medroxyprogesterone acetate 10 mg/d, 1 times/d,3 month was 1 treatment course, and 46 cases of the observation group by traditional Chinese medicine of tonifying kidney. Water decoction, 1 dose/ d, orally 2 times per day, continuous take three weeks, stop to begin the next treatment after a week, taking 3 consecutive months. To observe status of uterine and ovariano, the serum follicle stimulating hormone （ FSH）, luteinizing hormone （LH） , estradiol（E2） determination by radioimmunoassay, and CD4~ and CD8 ~. T lymphocyte subsets by flow cytometry before and after treatment. Results The endometrial thickness of the observation group after treatment was （O. 51 ± O. lO）cm,higher than that of the control group after the treatment of （0.44 ±0.07） era,the difference was statistically significant （ t = 4.17, P 〈 0.05 ）. After treatment FSH and LH in the observation group were （ 36.08 ± 1.37 ） U/L and （34.63 ± 2.05 ）U/L,lower than the control group of （44.12 ± 1.05 ）U/L and （39.14± 1.88 ）U/L（ t = 5.19,4.99 ,P 〈 0.05）. E2 of the observation group was （ 75.89 ±6.74 ） pmol/L, which was higher than that of the control group （67.45 ± 6.28 ）pmol/L（ t = 5.38 ,P 〈 0.05 ）. Before treatment ,T lymphocytes of the control group and observation group were 30.68 ± 3.79 and 31.02 ± 3.16, after treatment were 34.03± 4.17 and 37.86 ± 4.38, the differences were statistically significant （ t = 5.24,4.91, P 〈 0.05 ）, and which of the observation group than in the control group （ t = 5.17, P 〈 O. 05）. After treatment,CD4＋ of the observation group was 47.11 ± 1.21 ,higher than that of the control group 40.42 ± 1.67 （ t = 5.80,P 〈 0.05 ）. After treatment, CD8＋ of the observation group was 31.06± 1.34,lower than that of the con- trol group 36.19 ± 1.73 （ t = 4.98, P 〈 0.05 ）. Conclusion Tonifying kidney of Chinese medicine could improve symptoms and the ovarian reserve function of patients with POF significantly, and regulate the levels of estrogen, and improve their immune function.
出处
《中华全科医学》
2014年第12期2004-2006,共3页
Chinese Journal of General Practice
基金
浙江省中医药科技计划项目(2008CB043)
关键词
补肾中药
卵巢早衰(POF)
性腺轴
免疫调节
Tonifying kidney
Premature ovarian failure（POF）
Gonadal axis
Immune regulation
作者简介
通讯作者：徐晓琴，E-mail：xxq1976@126．com
