摘要
目的采用组织培养快繁技术培养五指毛桃种苗,为人工种植提供种源。方法采用五指毛桃叶片作外植体,以MS和1/2 MS为基本培养基,采用正交设计研究植物生长调节剂多因素组合(6-BA、NAA、2,4-D、IAA、KT和IBA)对五指毛桃初代诱导、不定芽分化和诱导生根的影响。结果不定芽最佳诱导培养基为MS+1.0 mg/L 6-BA+0.3 mg/L NAA,外植体经20 d诱导培养,可分化形成不定芽72个;MS+1.0 mg/L 6-BA+0.3 mg/L IAA+0.3 mg/L KT最利于不定芽继代增殖,增殖倍数6.67;1/2 MS+1.0 mg/L IBA+0.3 mg/L NAA最适于诱导生根获得再生植株,生根率100%;宜移栽于泥炭-珍珠岩(1︰1)的基质上,成活率为93%。结论此途径繁殖速度快、再生率高,能为栽培五指毛桃提供大量种苗。
Objective To solve the shortage problem of large-scale planting the seedlings in the roots of Ficus hirta by rapid propagation in vitro tissue culture. Methods The MS and 1/2 MS media were used as basic media, The multi factor combination of plant growth regulator（6-BA, NAA, 2, 4-D, IAA, KT, and IBA） on the seedling subculture and root culture was studied by orthogonal design. Results The best medium for the adventitious bud induction was MS ＋ 6-BA 1.0 mg/L ＋ NAA 0.3 mg/L, 72 adventitious buds were obtained by differentiation with 20 d induction of explants; The best medium for cluster inducing and subculture was MS ＋ 1.0 mg/L 6-BA ＋ 0.3 mg/L IAA ＋ 0.3 mg/L KT; The best rooting medium was 1/2 MS ＋ 1.0 mg/L IBA ＋ 0.3 mg/L NAA, and the rooting rate was 100%. Being suitably Transplanted to peat-perlite（1：1） matrix, the 30 d survival rate was 93%. Conclusion The tissue culture for the roots of F. hirta could be used to produce test-tube seedlings for large scale planting.
出处
《中草药》
CAS
CSCD
北大核心
2014年第17期2547-2551,共5页
Chinese Traditional and Herbal Drugs
基金
广西壮族自治区卫生厅中医药科技专项(GZKZ10-081)
作者简介
李林轩（1986-），男，在职研究生，助理研究员，从事中药资源保护与开发利用研究。Tel：18577195096E—mail：starryll25@sina．com
通信作者韦坤华（1983-），女，博士，副研究员，从事药用植物生物技术研究。E-mail：divinekh@163．com
