摘要
目的：观察具清热化湿疏肝祛瘀作用的中药复方三黄柴术方对急性肝内胆汁瘀积大鼠炎症细胞因子ICAM-1、TNF-α的影响,探讨该复方防治肝内胆汁瘀积的可能的作用机制。方法：SD雄性大鼠随机分成正常组8只,模型组、三黄柴术方组及熊去氧胆酸组各24只,后3组又分24、48、72 h 3个时相点。三黄柴术方组及熊去氧胆酸组于第1～5天分别灌服三黄柴术方煎液5.0 g·kg^-1·d^-1及熊去氧胆酸90 mg·kg^-1·d^-1,第3天按100 mg·kg^-1灌服麻油配制的4%ANIT1次;正常组第1～3天每天均灌服等容量的蒸馏水,第3天灌服等量的麻油;模型组第1～5天每天均灌服等容量的蒸馏水,模型组第3天灌服麻油配制的4%ANIT1次。正常组于服用麻油后24 h全部处死,其余3组分别于灌服ANIT后24、48、72 h随机选取每组7～8只大鼠,空腹取血,收集肝脏标本。ELISA检测血清ICAM-1、TNF-a含量;免疫组织化学Envision法检测肝组织中ICAM-1蛋白的表达。结果：模型组大鼠血清ICAM-1在灌服ANIT 24、48、72 h后逐渐增高;血清TNF-α水平及肝组织ICAM-1表达在灌服ANIT 24 h后明显增高,48 h达到高峰,72 h下降;模型组大鼠3个时相点血清ICAM-1、TNF-α水平和肝组织ICAM-1表达均较正常组显著升高（P〈0.01、P〈0.05）;三黄柴术方组及熊去氧胆酸组在3个时相点血清ICAM-1、TNF-α水平和肝组织ICAM-1表达较模型组明显降低（P〈0.01,P〈0.05）。结论：三黄柴术方能明显抑制肝内胆汁瘀积大鼠肝组织ICAM-1过表达,降低血清炎症因子ICAM-1、TNF-α的水平,从而减轻肝细胞的损伤,这可能是其防治肝内胆汁瘀积的重要机制之一。
Objective：To investigate the effects of compound Sanhuang Chaishu Formula with soothing liver and resolving blood stasis and removing heat and dampness method on prevention from acute intrahepatic cholestasis and on basis of ICAM- 1 and TNF- α to explore their mechanism of prevention from acute intrahepatic cholestasis. Methods：Male sqrague- Dawley（SD） rats were randomly divided into 4 groups,8 rats in the normal group,24 rats respectively in the model,Sanhuang Chaishu Formula and UDCA groups. From 1stto 5thday,the rats in the treatment groups were administrated respectively with Sanhuang Chaishu Formula decoction（5. 0 g·kg^-1·d^-1） and UDCA（90 mg·kg^-1·d^-1） by gastric gavage. They were administrated with 4% ANIT prepared with sesame oil by gastric gavage once in the 3rdday. All the normal rats were administrated with the same volume of distilled water from the 1stto 3rdday,in the 3rdday. The rats were administrated with sesame oil. All the model rats were administrated with the same volume of distilled water from the1 stto 5thday. They also were administrated with 4% ANIT prepared with sesame oil by gastric gavage a time in the 3rd day. All the normal rats were decapitated after 24 hours of administration of sesame oil,all the others 7- 8 rats in the other groups were randomly selected and fasting venous blood and rats liver were obtained after 24 hours,48 hours,72 hours of administration of gastric ANIT. The serum ICAM- 1 and TNF- α were detected with ELISA. The protein expressions of ICAM- 1 were detected with the Envision method of immunohistochemistry. Results：After 24 hours,48 hours and 72 hours of administration of ANIT,serum ICAM- 1 in the model gradually increased. The protein expression of liver ICAM- 1 and serum TNF- α in the model gradually increased from 24 thhour to 48 thhour,at 48 thhour to the summit,at 72 ndhour,beganto decrease. At the three phase points serum ICAM- 1,TNF- α and the protein expressions of liver ICAM- 1 in the model group were much higher than those of the normal group.（ P〈0. 01,P〈0. 05）. After administration,at the three phase points serum ICAM- 1,TNF- α and the protein expression of liver ICAM- 1 in the Sanhuang Chaishu Formula and UDCA groups were much lower than those of the models（ P〈0. 01,P〈0. 05）. Conclusion：Compound Sanhuang Chaishu Formula can obviously restrain the over- expression of liver ICAM- 1 in intrahepatic cholestasis rats. Lower serum inflammatory factor ICAM- 1 and TNF- α sequentially relieve liver of injury. It may be one of the most important mechanisms for compound Sanhuang Chaishu Formula to prevent ntrahepatic cholestasis.
出处
《中华中医药学刊》
CAS
2014年第10期2428-2430,I0011,共4页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省中医药科技计划青年项目(2009YB031)
关键词
胆汁瘀积
清热化湿疏肝祛瘀法
胞间黏附分子-1
肿瘤坏死因子-Α
cholestasis
soothing liver and resolving blood stasis and removing heat and dampness method
intercellu-lar adhehesion molecule-1
tumor necrosis factor-α
作者简介
蔡丹莉（1978-），女，浙江诸暨人，主治医师，研究方向：中西医结合防治消化系统疾病及急危重症的研究。
