摘要
目的分析本实验室抗核抗体(ANA)与抗核抗体谱(ANAs)17项联合检测的情况,并探讨两者之间的相关性。方法分别用间接免疫荧光法和免疫印迹法检测患者ANA和ANAs,判断其ANA核型和ANAs结果,并对各荧光核型和各种特异性抗核抗体结果进行统计分析。结果 340例ANA阳性患者标本中,颗粒型214例,着丝点型51例,胞浆颗粒型28例,核仁型25例,均质型17例,周边型5例,颗粒型是检出的主要核型。ANAs 17项抗体阳性率为82.94%。214例荧光颗粒型标本中,常见的抗体为抗SSA/Ro52kd(31.78%)、抗SSA/Ro60k(d30.84%)和抗SSB/La(19.16%)抗体;17例荧光均质型标本中,常见的抗体为抗核小体(47.06%)和抗组蛋白(41.18%)抗体;抗CenpB对着丝点核型的诊断具有较高的特异性,阳性率为92.16%(47/51)。结论不同荧光核型的ANA与ANAs抗体之间有一定的关系,但并无绝对的规律可寻,两种ANA的检测方法,间接免疫荧光法和免疫印迹法联合应用,有助于自身免疫性疾病(AID)的诊断和预后观察。
Objective To analyze the results of the 17-index combined detection of antinuclear antibodies （ANA）karyotypes and antinuclear antibody spectrum （ANAs）in our laboratory and to investigate the correlation between them.Methods ANA and the specific ANAs were detected by the indirect immunofluorescence（IIF）and the immunoblotting respectively.The results of the ANA karyotypes and the specific ANAs were statistically analyzed.Results Results Among 340 samples of ANA positive,214 cases were the speckled pattern,51 cases were the centromere pattern,28 cases were the cytoplasmic pattern,25 cases were the nucleolar pattern,17 samples were the homogeneous pattern and 5 samples were the peripheral pattern,the speckled pattern was the main de-tected pattern.The positive rate of 17-index ANAs was 82.94%.In 214 cases of speckled pattern,the common antibodies were anti SSA/Ro52kd （31.78%）,anti-SSA/Ro60k （d30.84%）and anti-SSB / La （19.16%）;in 17 cases of homogenous pattern,the common antibodies were anti-nucleosome （47.06% ）and anti-histone （41.18% ）;anti-CenpB was specific to pattern of centro-mere,the positive rate was 92.16% （47/ 51 ）.Conclusion There is a certain relationship between different fluorescent patterns and ANAs,but there is no absolute rule,so IIF and immunoblotting as two kinds of ANA detection methods should combined to use,which will contribute more to the diagnosis and prognosis observation of autoimmune disease（AID）.
出处
《国际检验医学杂志》
CAS
2014年第14期1859-1860,1863,共3页
International Journal of Laboratory Medicine
关键词
抗核抗体
抗核抗体谱
间接免疫荧光法
免疫印迹法
antinuclear antibody
antinuclear antibody spectrum
indirect immunofluorescence
immunoblotting
作者简介
陈燚琼，女，主管技师，硕士，主要从事自身免疫性疾病的实验诊断研究。通讯作者：E-mail：lizhsh2001@163．com。
