摘要
[目的]为动物组织中氟苯尼考残留快速检测试纸条的制备奠定基础。[方法]采用琥珀酸酐法制备氟苯尼考人工半抗原,用活泼酯法将氟苯尼考人工半抗原与牛血清蛋白进行偶联制备氟苯尼考人工抗原。紫外光谱法和光谱分析法对氟苯尼考人工抗原FFCHS-BSA进行鉴定。[结果]与BSA和FFC相比,FFC-HS-BSA的最大吸收峰发生了变化,曲线形状也发生了改变,FFC-HS与BSA的偶联比为18.9∶1。[结论]FFC-HS-BSA人工抗原的合成是成功的,其偶联比在最适范围内。
[Objective] To lay the foundation for the preparation of animal tissues florfenicol residue test strip for rapid detection. I Method ] Florfenicol antigen was synthesized by using the suceinie anhydride method. Florfenicol synthesized antigen were prepared with the active ester method florfenicol artificial hapten conjugated to bovine serum proteins. Florfenieol synthesized antigen were identified by UV spectroscopy and spectroscopic analysis. [ Result] FFC-HS-BSA compares with BSA and FFC, the maximum absorption peak of the FFC-HS-BSA changed. The shape of the curve of FFC-HS-BSA also changed. FFC-HS and BSA coupling ratio is 18.9：1. [ Conclusion] Synthesis of FFC-HS-BSA artifi- cial antigen was successful, the coupling ratio was within its optimum range.
出处
《安徽农业科学》
CAS
2014年第23期7757-7758,共2页
Journal of Anhui Agricultural Sciences
基金
安徽科技学院自然科学研究项目(ZRC201425)
关键词
氟苯尼考
人工抗原
琥珀酸酐法
活泼酯法
偶联比
Florfenicol
Synthesized antigen
Succinic anhydride method
Active ester method
Coupling ratio
作者简介
孙素玲（1958-），女，安徽砀山人，教授，从事兽药残留检测的研究。
