摘要
采用活泼酯法将三唑磷半抗原(TZPM-Hap)与牛血清蛋白(BSA)和卵血清蛋白(OVA)偶联,制备出免疫抗原(TZPM-A-BSA)和包被抗原(TZPM-A-OVA),经紫外扫描证明人工抗原合成成功。用免疫抗原TZPM-A-BSA免疫雌性Balb/c小鼠,获得了特异性的三唑磷多克隆抗体。以所获得的抗体建立间接竞争酶联免疫吸附分析法,三唑磷多抗的IC50值为0.01μg/mL;交叉反应表明,三唑磷的特异性多抗与其他有机磷农药结构类似物无明显的交叉反应。
In this paper, artificial antigens for tdazophos including one immunogen and one coating antigen were prepared. The hapten （TZPM-Hap） of triazophos was coupled to bovine serum albumin （BSA） and ovalbumin （OVA） by the active ester method to prepare the artificial immunogen TZPM-A-BSA and the coating antigen TZPM-A-OVA, respectively. Immunogen and coating antigen were successfully prepared and confirmed by ultraviolet spectrometry. Polyclonal antibodies against triazophos were produced from female Balb/c mice, which were immunized with the artificial immunogen TZPM-A-BSA. Indirectly competitive ELISA was established with this antiserum, of which IC50 was 0.01 μg/mL. The polyclonal antibodies showed no obvious cross-reactivity with other structural-related organophosphate pesticides.
出处
《现代食品科技》
EI
CAS
2011年第12期1469-1471,共3页
Modern Food Science and Technology
关键词
三唑磷
活泼酯法
人工抗原
多克隆抗体
酶联免疫吸附检测
triazophos
active ester method
artificial antigen
polyclonal antibody
enzyme-linked immunosorbnent assay
