摘要
目的观察马鞭草总黄酮对大鼠脓毒性急性肺损伤(ALI)的保护作用和机制。方法将120只大鼠随机分为6组,分别为假手术组(0.9%氯化钠溶液),模型组(0.9%氯化钠溶液),地塞米松阳性对照组(2.0 mg·kg-1,ip),马鞭草总黄酮低、中、高剂量组(50.0,100.0,150.0 mg·kg-1,ip),盲肠结扎穿孔法(CLP)建立脓毒症诱发ALI大鼠模型,造模24 h后给药,48 h后处死,观察支气管肺泡灌洗液(BALF)中白细胞、蛋白含量和肺湿/干质量比(W/D),比较炎症递质水平、抗氧化作用以及肺组织核转录因子(NF-κB)p65表达和细胞凋亡率。结果马鞭草总黄酮能显著降低BALF中白细胞、蛋白含量和肺组织W/D(P<0.05或P<0.01),降低巨噬细胞炎症蛋白-2(MIP-2)、细胞间黏附分子-1(ICAM-1)、肿瘤坏死因子-α(TNF-α)的水平(P<0.05或P<0.01)以及肺组织细胞凋亡率和NF-κB p65表达(P<0.05或P<0.01),改善丙二醛(MDA)含量、超氧化物歧化酶(SOD)和髓过氧化物酶(MPO)活性(P<0.05或P<0.01)。尤其高剂量组效果明显(P<0.01)。结论马鞭草总黄酮能降低脓毒性急性肺损伤大鼠的炎症递质水平,减轻脂质过氧化,改善肺泡毛细血管通透性,缓解ALI的发生、发展。
Objective To observe the protective effect and mechanism of the total flavonoids of verbena on rats withseptic acute lung injury （ ALI）. Methods A total of 120 rats were randomly divided into sham operation group （0. 9%sodium chloride solution ）, model group （ 0. 9% sodium chloride solution ）, dexamethasone positive control group （2. 0 mg·kg-1 , ip）, low, middle and high dose of the total flavonoids of verbena group （50. 0, 100. 0, 150. 0 mg·kg-1 , ip）.Rat model with ALI was induced by Cecal ligation and puncture （ CLP）. The rats were injected with physiological saline,dexamethasone, low, middle and high dose of the total flavonoids of verbena, respectively 24 h after establishment of the ALImodel, and executed 48 h later. Bronchoalveolar lavage fluid （BALF） changes in leukocyte, protein content and the lung tissuewet to dry weight ratio （ W/ D） were observed, and the levels of inflammatory mediators, oxidation stress and nucleartranscription factor （NF-κB） p65 expression and cell apoptosis in lung tissue were compared. Results Total flavonoids of verbena significantly decreased leukocytes, protein content and W/ D in BALF （ P〈0. 05 or P〈0. 01）, decreased microphageinflammation protein-2 （MIP-2）, intercellular adhesion molecule-1 （ICAM-1）, tumor necrosis factor-α（TNF-α） and apoptoticrate of lung cells and expression of NF-κB p65 （ P 〈0. 05 or P 〈0. 01）, and improved MDA content, activity of superoxidedismutase （SOD） and myeloperoxidase （MPO） （P〈0. 05 or P〈0. 01）. The effects were the greatest in the high dose group （P〈0. 01）. Conclusion The total flavone of verbena can reduce the levels of inflammatory mediators, reduce lipid peroxidation,improve the alveolar capillary permeability, and alleviate the occurrence and development of ALI.
出处
《医药导报》
CAS
北大核心
2014年第4期429-433,共5页
Herald of Medicine
关键词
马鞭草总黄酮
损伤
肺
急性
脓毒性
保护机制
Total flavonoids from verbena
Injury, lung, acute
Septic
Protection mechanism
作者简介
宋兆华（1981-）,男,河南漯河人,讲师,硕士,从事人体解剖学教学与研究.电话（0）13938013804,E-mail：szhualh@163.com.
