摘要
目的探讨血管活性肠肽(VIP)对脓毒性休克大鼠的保护作用及其可能机制。方法采用盲肠结扎加穿孔(CLP)法制备脓毒性休克大鼠模型。将30只雄性 SD 大鼠随机分成3组:假手术组(SO,n=10)、CLP 组(n=10)和 VIP 组(n=10)。VIP 组大鼠在行 CLP 术后即刻给予5 nmolVIP,持续监测各组大鼠的平均动脉压(MAP),统计20 h 存活率;应用酶联免疫吸附试验(ELISA)检测血清肿瘤坏死因子-α(TNF-α)、白介素-10(IL-10)水平;取大鼠肺、肾和肠等组织行病理检查。结果在8 h 以后的各时间点,VIP 组的 MAP 显著高于 CLP 组(P<0.05);与 CLP 组比较,VIP 组的TNF-α水平明显降低、IL-10水平明显升高,20 h 存活率显著提高(P<0.05),各脏器病理损伤明显改善。结论 VIP 通过抑制促炎因子的生成并促进抗炎因子的产生在大鼠脓毒性休克中发挥保护作用。
Objective To investigate the protective effects of vaseactive intestinal peptide （VIP） on septic shock rats and explore its possible mechanism. Methods Cecal ligation and puncture （CLP） was performed to produce septic shock rat model. Thirty adult Sprague-dawley rats were randomly divided into 3 groups with 10 animals in each group：sham operation group, CLP group and VIP group. The rats in VIP group were given intravenous injection of VIP（ 5 nmol per rat） instantly after the CLP operation. Then the mean arterial pressure （MAP） was monitored consistently and survival rate was observed. Blood samples were obtained from femoral artery for measuring the serum concentrations of tumor necrosis factor-or （ TNF-α） and interleukin-10 （IL-10） by using enzyme-linked immunosorbent assays （ELISA）. Organs （lung, kidney and intestine） were harvested for pathological examination. Results At each time point after 8 h, the MAP of VIP rats was significantly higher than that in CLP rats （ P 〈 0. 05 ）. In VIP group rats, the serum TNF-α concentration was decreased meanwhile IL-10 level was increased with markedly alleviated organic pathological injuries and the survival rate was obviously raised. Conclusions VIP exerts protective effects on septic shock rats through inhibiting production of proinflammatory factors and stimulating the production of anti-inflammatory cytokines.
出处
《中华外科杂志》
CAS
CSCD
北大核心
2007年第3期196-199,共4页
Chinese Journal of Surgery
作者简介
通讯作者：吴志勇，Email：zhengwk@online．sh．cn
