摘要
目的建立以聚酰胺薄膜为固定相,微乳液为展开剂,同时鉴别2种洋甘菊中5种化合物木犀草素、芹菜素、木犀草素-7-葡萄糖苷、芹苷元-7-葡萄糖苷、7-甲氧基香豆素的方法。方法以SDS-正丁醇-正庚烷-水微乳液为展开剂,并配以甲酸为有机改性剂,以2-氨基乙基联苯基硼酸酯、聚乙二醇400甲醇溶液为显色剂,考察了不同微乳液对两种洋甘菊甲醇提取物分离度的影响,通过调节展开剂的组成,寻找出最佳的薄层分离条件并分离鉴别了母菊、罗马洋甘菊。结果微乳液-甲酸(9∶2)为适宜的展开剂,其中微乳液SDS-正丁醇-正庚烷质量比为0.27∶0.63∶0.10、含水量75%。与硅胶薄层比较,分离效果明显提高。结论建立了一种分离鉴别母菊、罗马洋甘菊中木犀草素、芹菜素、木犀草素-7-葡萄糖苷、芹甙元-7-葡萄糖苷、7-甲氧基香豆素5种化合物的方法。方法简便、快速和准确。
Objective To investigate the application of microemulsion TLC to separation and identification of luteolin, apigenin, galuteolin,apigenin - 7 - glucoside, 7 - methoxycoumarin in two species of Chamomiles based on polyamide film as stationary phase. Methods SDS- n- butyl alcohol- n- heptane -aqueous microemulsion as the development in combination with methyl acid as the modifier and diphenylboric acid aminoethyl ester, macrogol 400 as spray reagent, the influence of the change in compo- nents of microemulsion on the resolution was examined to search for the optimal separation condition of methanolic extracts from Chamomile and Roman chamomile in Xiujiang. Results The best mobile phase was the microemulsion containing SDS - n - butyl alcohol - n - heptane （0.27：0.63： 0.1 ） ,75% water - methyl acid （ 9： 2）. As compared with conventional TLC, resolution of microemulsion TLC improved markedly. Conclusion Microemulsion TLC is an efficient, simple and new method for separating and identifying luteolin, apigenin, galuteolin, apigenin - 7 - glucoside ,7 - methoxycoumarin in two Species of Chamomiles.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2014年第7期1643-1645,共3页
Lishizhen Medicine and Materia Medica Research
基金
新疆维吾尔自治区自然科学基金(No.2012211A024)
作者简介
韩松林（1984-），男（汉族），新疆阿克苏人，阿克苏职业技术学院讲师，硕士学位，主要从事分析测试技术与新药研发工作．
李新霞（1968-），女（汉族），新疆乌鲁木齐人，新疆医科大学教授，硕士研究生导师，博士学位，主要从事分析测试技术与新药研发工作．
