摘要
目的建立茵芩合剂的质量控制方法。方法采用微乳薄层色谱法对制剂中的茵陈、黄芩和大黄进行鉴别;采用高效液相色谱法同时测定绿原酸、咖啡酸、黄芩苷和黄芩素的含量,色谱柱为Diamonsil C_(18)(2)(250 mm×4.6 mm,5μm),流动相为乙腈-0.25%磷酸溶液,梯度洗脱,流速为1.0 ml/min,检测波长为310 nm,柱温为30℃。结果微乳薄层色谱显色清晰且阴性对照无干扰;绿原酸、咖啡酸、黄芩苷和黄芩素分别在5.96~59.60μg/ml(r=0.9991)、0.60~6.00μg/ml(r=0.9992)、40.80~408.00μg/ml(r=0.9993)、1.98~19.84μg/ml(r=0.9994)范围内线性关系良好;平均加样回收率分别为96.56%、101.84%、98.77%和98.98%。结论本法操作简便、结果准确,重复性好,可用于茵芩合剂的质量控制。
Objective To develop a quality control method for Yinqin Mixture.Methods Artemisiae Scopariae Herba,Scutellareae Radix,and Rhei Radix et Rhizoma.were identified by microemulsion thin layer chromatography.Chlorogenic acid,caffeic acid,baicalin,and baicalein were simultaneously determined by high performance liquid chromatography,chromatographic column was Diamonsil C_(18)(2)column(250 mm×4.6 mm,5μm),the mobile phase consisted of acetonitrile-0.25%phosphoric acid solution with gradient elution at a flow rate of 1.0 ml/min,the detection wavelength was 310 nm and the column temperature was 30℃.Results The color of microemulsion thin layer chromatography was clear and the negative had no interference;the linear range of chlorogenic acid,caffeic acid,baicalin,and baicalein were 5.96-59.60μg/ml(r=0.9991),0.60-6.00μg/ml(r=0.9992),40.80-408.00μg/ml(r=0.9993),1.98-19.84μg/ml(r=0.9994),respectively;average recovery rates of components were 96.56%,101.84%,98.77%,and 98.98%,respectively.Conclusion The method is simple,accurate,good reproducibility,and can be used for the quality control of Yinqin mixture.
作者
欧人豪
曾金
韦祖巧
李萍
OU Renhao;ZENG Jin;WEI Zuqiao;LI Ping(Department of Pharmacy,Liuzhou Maternity and Child Healthcare Hospital(Liuzhou Maternity Hospital,Liuzhou Children’s Hospital),Guangxi Zhuang Autonomous Region,Liuzhou 545001,China)
出处
《中国医药导报》
CAS
2022年第3期29-33,共5页
China Medical Herald
基金
广西壮族自治区卫生和计划生育委员会自筹经费科研课题(Z20170522)。
关键词
茵芩合剂
微乳薄层色谱法
高效液相色谱法
绿原酸
咖啡酸
黄芩苷
黄芩素
含量测定
Yinqin Mixture
Microemulsion thin layer chromatography
High performance liquid chromatography
Chlorogenic acid
Caffeic acid
Baicalin
Baicalein
Content determination
作者简介
欧人豪(1974.12-),男,硕士,副主任药师,主要从事医院制剂生产与研发工作。
