摘要
本试验旨在研究检测牛奶中林可霉素(lincomycin,LIN)胶体金免疫层析试纸条。根据直接竞争抑制和间接竞争抑制2种模式,通过对金标包被原(抗体)量、pH、包被抗体(抗原)浓度、牛奶前处理等试验的优化,制备出2种满足实际检测需要的胶体金试纸条;二者的检测限分别为30和10ppb。在实际检测中,间接法在灵敏度、稳定性和检测时间等方面都优于直接法。
The objective of this study was to develop gold immunochromatography test strips to detect lincomycin in milk.Based on two patterns of direct competitive inhibition and indirect competitive inhibition, we optimized concentration of immunogold labeling BSA-LIN （IgG）, pH, concentration of coating IgG （BSA-LIN）, pretreatment of milk and so on. The resultsshowed that we got two strips that fulfil practical requirement. The LOD of direct and indirect were 30 and 10 ppb. Comparingtwo methods, indirect method was better than direct in sensitivity, stability and testing time.
出处
《中国畜牧兽医》
CAS
北大核心
2014年第7期159-163,共5页
China Animal Husbandry & Veterinary Medicine
基金
公益性行业(农业)科研专项经费项目(201203069-2)
奶牛产业体系北京市奶牛创新团队资金
作者简介
周洁（1989-），女，内蒙古人，硕士生，研究方向：兽药残留检测。
通信作者：丁双阳（1966-），女，研究员，主要从事动物源性食品残留关键技术及标准研究。Tel：010-62732802；E-mail：dingsryr@cau．edu．Cn
