摘要
目的考察金银花药物成分经正常细胞及感染流感病毒细胞代谢后,金银花主要成分的变化情况。方法应用中药指纹图谱技术,将得到的色谱图导入指纹图谱相似度评价系统进行分析,比较各组间的标准指纹图谱主要特征色谱峰,找出与金银花抗流感作用相关的差异色谱峰。结果以培养基空白组为对照,正常细胞给药组、病毒细胞给药组各主要特征峰的峰面积表现出整体下降趋势。8号峰面积明显下降,2号峰、7号峰与c号峰与培养基空白组及金银花对照药材比较,相对峰面积成倍增加。结论 2、7、8、c号峰对应的化合物可能与金银花抗流感病毒活性密切相关。
Objective To investigate the main composition changes of Fols lonicerae through the metabolism of normal cells and cells infectedby influenza virus.Methods Applied with the fingerprint technology,and imported the obtained chromatograms into fingerprint similarity evaluation system component,and analyzed.Compared the main characteristic peaks of the standard fingerprints among groups to find out the different chromatographic peaks related to the effect of Flos lonicerae anti-influenza.Results The blank group as control,the main characteristic peaks area of the normal cells group and cells group infected by virus showed a general downward trend.Compared with blank group and the control medicine Flos Lonicerae,No.8 peak area decreased significantly,No.2,No.7 peak and peak c,relative peak area multiplied.Conclusion The compounds of 2,7,8,c peaks corresponding may be closely related to the anti-influenza virus activity of Flos lonicerae.
出处
《药学研究》
CAS
2014年第7期376-378,共3页
Journal of Pharmaceutical Research
基金
山东省中医药科技发展计划项目(No.2011-162)
山东省自然科学基金项目(No.Y2007C177)
关键词
金银花
抗病毒
细胞代谢
Flos lonicerae
Antiviral
Cell metabolism
作者简介
郭承军，男，博士研究生，副主任中药师，研究方向：运动损伤治疗的中药新药，E-mail：guochj28@126．com.
张会敏，女，博士研究生，助理研究员，研究方向：中药化学、中药炮制及中药质量标准，Tel：0531—82949830，E-mail：huiminzhang@163．com.
