摘要
利用乙醇为溶剂提取桂花中总黄酮类物质.通过单因素实验和正交试验确定桂花总黄酮最佳提取工艺条件,并对桂花总黄酮体外抗氧化性能进行系统评价.得出以乙醇为溶剂提取桂花中总黄酮最佳工艺条件为:乙醇体积分数60%,提取功率360 W,料液比1∶35,浸提时间2 min,在此条件下总黄酮得率可达18.18%;体外抗氧化性能研究结果表明,桂花总黄酮具备较好抗氧化活性,在相同的加入量下桂花总黄酮对DPPH自由基清除率明显优于BHT,低于Vc.
Ethanol is used as the solvent to extract the total flavonoids from the osmanthus＇ flavor with the microwave -assisted extraction. The single -factor experiments and orthogonal test are used to determine the best extraction process of the osmanthus＇ total flavonoids, and systemic evaluation is used to determine the in -vitro antioxidation property of the osmanthus＇ total flav0noids. The experimental results have showed that the optimum extraction conditions for the total flavonoids of the osmanthus＇ residua with ethanol as the solvent are as follows ：the ethanol concentration is 60%, the extraction power is 360 W, the material/extraction solution ratio 1 ： 35 and the extraction time is 2 min. Under the above optimum conditions, the total flavonoids＇ yield was up to 18.18%. The experimental results of the in - vitro antioxidation property have showed that the total flavones of osmanthus have better antioxidant ability, adding osmanthus＇ total flavonoids. With the same quantity, it was obviously higher than that of BHT under the condition of sweet scented Osmanthus fragrans flavonoids on DPPH free radical, and less than Vc.
出处
《云南民族大学学报（自然科学版）》
CAS
2014年第4期239-242,共4页
Journal of Yunnan Minzu University:Natural Sciences Edition
基金
武夷学院对接南平市产业发展科研专项项目(2011DJ10)
关键词
桂花
总黄酮
提取工艺
抗氧化
Osmanthus fragrans
total flavonoids
extraction
antioxidant
作者简介
陈培珍（1968-），女，硕士，副教授，主要研究方向：天然产物。
