摘要
采用正交试验法对超临界CO2 萃取连翘挥发油的条件进行了研究 ;结果显示最佳萃取条件为萃取压力30MPa ,萃取温度35℃ ,解析压力6.7MPa,解析温度50℃ ,粉碎度0.55mm ;按对结果的影响大小依次排列为萃取温度 ,粉碎度 ,萃取压力 ,解析压力 ,解析温度 ;用气相色谱 -质谱联用技术测定了最佳萃取条件所得连翘挥发油的化学成分 ,从中鉴定出21种成分 ,并测定了其相对含量 ,主要成分为α_蒎烯、β_蒎烯、萜品醇_4、α_萜品醇、苯甲醇等。
Orthogonal design of 5 factors at 3 levels was applied for optimization of supercritical CO2 fluid ex_traction conditions of essential oil from Fructus Forsythiae. The optimal conditions of extraction pressure 30 MPa, extraction temperature 35 ℃, separate pressure 6.7 MPa, separate temperature 50 ℃, and crush degree 0.55 mm were obtained. The sequence according to the effect of the extraction results is as follows: extraction temperature, crush degree, extraction pressure, separate pressure, separate temperature. Constituents of the essential oil were analyzed by gas chromatography-mass spectrometry, and 43 compounds were separated, 21 compounds of them were identified, their relative contents were determined by normalization method of areas. The main constituents were found to be α- pinene, β- pinene, terpineol- 4, α- terpineol, benzyl alcohol, etc.
出处
《分析测试学报》
CAS
CSCD
北大核心
2002年第4期34-37,共4页
Journal of Instrumental Analysis
基金
广东省科技攻关项目(2KB01202S)
