摘要
目的探讨养血安神片中梓醇与毛蕊花糖苷的含量测定方法,为进一步对药物做出评价而提供科学有效的依据。方法利用HPLC法进行养血安神片的梯度洗脱,色谱柱为AgilentSB-AQ(4.6 mm×250 mm,5μm),流速1.0 mL·min-1,检测波长为210 nm,柱温30℃。结果养血安神片中梓醇与毛蕊花糖苷成分均表现出良好的线性,达到基线分离的要求。结论该方法准确可靠、灵敏、重现性好,适合养血安神片中梓醇与毛蕊花糖苷含量的测定。
Objective To study the determination method about the catalpol and verbascoside,provide basis for quality evaluation.Method Gradient elution was applied in HPLC analysis. Agilent SB-AQ chromatographic Column( 4. 6 mm × 250 mm,5 μm),velocity 1. 0 mL·mim- 1,column temperature was 30℃,wavelength was 210 nm. Result Two components can achieve good baseline separation,linear. Conclusion After the syste maticmethodology,the method is simple,accurate,reproducible,applicable to the provisions by pharmacy opoeia rehmannia glutinosa two content determination.
出处
《安徽医药》
CAS
2014年第10期1859-1861,共3页
Anhui Medical and Pharmaceutical Journal
