摘要
目的建立RP-HPLC同时测定肉苁蓉中松果菊苷和毛蕊花糖苷的含量.方法采用HP-1100色谱系统和YMC-PackODS-A柱(5μm,25mm×4.6 mm);以CH3CN-MeOH-1%HAc(10:15:75)为流动相,流速0.7mL·min-1,检测波长为334nm,柱温:30℃.结果松果菊苷进样量在0.12～1.47μg内、毛蕊花糖苷在0.18～2.16μg内与峰面积呈良好线性关系,相关系数均为0.9999;平均回收率分别为98.87%和97.01%.结论本方法简便,精密度、重现性良好,结果准确可靠.可用于肉苁蓉药材、饮片及其制剂的含量测定.
OBJECTIVE: To establish a RP-HPLC method for the simultaneous determination of echinacoside and acteoside in Herbe Cistanches. METHODS: HP - 1100 HPLC system and YMC - Pack ODS-A column(5 μm, 4.6 mm × 250 mm) were adopted. The mobile phase consisted of CH3CN-MeOH-1% HAc(10:15:75) and the detection wavelength was 334 nm with a PDA detector. The flow rate was 0.7 mL· min-1 and the column temperature was 30°C. RESULTS: The linear ranges of echinacoside and acteoside were 0.12-1.47 μg (r = 0.999 9) and 0.18-2.16 μg (r = 0.999 9), respectively. The average recoveries(n = 5) of echinacoside and acteoside were 98.87% (RSD% = 1.85) and 97.01% (RSD% = 0.97) respectively. CONCLUSION: This method is simple, accurate and reliable. It can be used as a quantitative determination method for the quality control of Herbe Cistanches and its preparation.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2004年第10期740-741,共2页
Chinese Pharmaceutical Journal
基金
中央级科研院所科技基础性工作专项
关键词
肉苁蓉
松果菊苷
毛蕊花糖苷
高效液相色谱法
Herbicides
High performance liquid chromatography
Quality control
Thermal effects
