摘要
目的:观测血管紧张素Ⅱ（Angiotensin Ⅱ,Aug Ⅱ）对体外培养的牛眼小梁细胞（bovine trabecularmeshwork cells,TM cell）3H胸腺核苷酸（3H-hymidine,3H-TdR）掺入率及胶原合成的影响,探讨原发性青光眼的发病机制。方法:（1）牛眼小梁细胞的体外培养:应用免疫组化方法（neuronal specific enolas,NSE,Ⅷ因子相关抗原染色）、细胞鉴定、光学及电子透射显微镜对细胞进行形态学及生长特性的观察;（2）Aug Ⅱ（1×10-7mol·L-1及 1×10-8mol·L-1）以及其Ⅰ型受体（angiotensin receptor type I,AT1）拮抗剂孵育TM细胞,采用检测3H-TdR掺入率的方法了解细胞增殖状态,并用化学方法检测培养液中羟脯氨酸含量,间接推导胶原含量。结果:牛眼小梁细胞培养成功,以上皮型为主。Ang Ⅱ明显地增加了牛眼小梁细胞的3H-TdR摄入 率,同时培养液中的羟脯氨酸含量亦相应增高。结论:体外培养牛眼小梁细胞技术是研究小梁细胞特性的重要实验技术。AngⅡ可以诱导体外培养的牛眼小梁细胞的细胞增殖率升高,同时促使胶原合成增强。AT1受体拮抗剂可以部分阻断此促增殖效应。眼科学报2001;17:209～212。
Objective: To investigate the effects of angiotensin Ⅱ (Ang Ⅱ) on the cell proliferation and collagen synthesis of bovine trabecular meshwork (TM) cells in vitro. It may probe into the mechanism of primary open-angle glaucoma (POAG).Methods: (1) The bovine trabecular meshwork cells were cultured and identified by immunohistochemistry method (neuronal specific enolas , NSE, factor related antigan) . The growing characteristics and morphological feature of cultured primary and passaged cells were observed by invered microscope and eletron-microscope; (2) Ang Ⅱ(1× 10-7 mol.L-1 and 1 ×10-8mol . L-1) and angiotensin receptor type I (AT1) antagonist (losartan) were incubated with cultured TM cells. The cellular proliferation was measured by 3H-thymidine(3H-TdR) incorporation assay and collagen synthesis was indirectly professed though detects the hydroxyproline of medium with chemistry methods.Rusults: The cells of bovine trabecular meshwork were cultured successfully. Most of them were epithelia type. Ang Ⅱ increased the TM cells uptake of 3H-TdR and inhibited by losartan partially. Meanwhile the hydroxyproline of medium was increasedcorrespondingly.Conclusion: Establishing the method of culturing bovine trabecular meshwork cells is an important method for researching the characteristics of TM cells. Ang Ⅱ can induce the cell proliferation of bovine TM cells and increase the synthesis of collagen in vitro. The AT1 antagonist may inhibit this cell proliferation effect. Eye Science 2001; 17: 209 ~ 212.
出处
《眼科学报》
CAS
2001年第4期209-212,共4页
Eye Science
基金
西安交通大学第一医院科研基金(编号:YK9906)资助
