摘要
目的研究NF-κB对人眼小梁细胞基质金属蛋白酶-3(matrixmetalloproteinase-3,MMP-3)及组织金属蛋白酶抑制剂-1(tissueinhibitorofmetalloproteinase-1,TIMP-1)mR-NA表达的影响。方法采用原代培养的人眼小梁细胞,通过脂质体转染的方法,将P65表达质粒转染小梁细胞,24h后通过RT-PCR方法检测MMP-3及TIMP-1mRNA的表达。结果P65转染后MMP-3mRNA的表达明显增高,与正常对照组相比差异有显著性(P<0·05),TIMP-1mRNA的表达与正常对照组相比无明显改变(P>0.05)。结论NF-κB的活化可激活MMP-3的表达,提示NF-κB信号通路参与了小梁网外引流通道的调控。
Objective To study the effects of NF-κB on expression of matrix metalloproteinase-3 （ MMP-3 ） and tissue inhibitor of metalloproteinase-1 （TIMP-1 ） mRNA in human trabecular meshwork cells（ HTM cells）. Methods Lipofectin method was used to transfect P65 plasmid into the primary cultured HTM cells. Twenty-four hours after transfection, the expression of MMP-3 and TIMP-1 mRNA were measured by RT-PCR method. Results The expression of MMP-3 mRNA was dramatically increaser than the control group（P 〈0.05） after transfection,the expression of TIMP-1 mRNA had no significant difference with those in the control group （P 〉 0.05 ）. Conclusion The activation of NF-κB can increase the expression of MMP-3 mRNA in HTM cells, which suggests that the signal transduction pathways of NF-κB may participate in the regulation of aqueous humor outflow facility.
出处
《眼科新进展》
CAS
2007年第1期34-36,共3页
Recent Advances in Ophthalmology
作者简介
袁洪峰，男，1969年6月出生，云南大理人，博士。联系电话：023-68757341（O）；E—mail：yhf871@yahoo．com．cn
