摘要
目的 研究镉的雌性性腺毒性及其机制 ,为镉中毒防治研究提供科学依据。方法 不同剂量对雌性大鼠行亚慢性皮下注射染毒 (每天 1次 ,每周 5d ,连续 6周 )。染毒 2 0d后连续观察并记录动情周期 ;于染毒结束时进行促性腺激素释放激素 (GnRH)刺激试验 ,采用放免法测定FSH、LH变化 ;采用原子吸收光谱法测定卵巢、子宫、血清镉含量 ;将小鼠随机分成 3组 ,连续染镉 5d ,每天 1次 ,染毒第 6天进行超排卵试验。结果 ( 1)染毒组大鼠子宫、卵巢镉含量均高于低剂量组和对照组 (P <0 0 5 )且子宫镉与卵巢镉、血清镉含量有明显相关性 ;( 2 )高剂量组大鼠动情间期和动情周期异常率较其他剂量组明显增高 (P <0 0 5 ) ;( 3)注射GnRH前 ,各剂量组间大鼠血清中LH、FSH差异未见显著性 (P <0 0 5 ) ,注射GnRH后 ,各剂量组血清中LH、FSH水平较注射前均明显升高 ,差异有显著性 (P <0 0 5 ) ,而注射后染镉组大鼠血清中LH水平显著低于对照组 (P <0 0 5 ) ,FSH则否 ;( 4 )染镉小鼠超数排卵试验各组小鼠的卵子数差异无显著性 (P >0 0 5 )。结论 子宫、卵巢也是镉的重要靶器官 ,镉可引起雌性大鼠动情周期明显异常 ,染镉大鼠垂体对超量GnRH的反应能力和代偿功能明显损害 ,而卵巢对于超量促性腺激素的反应机能基本正常 。
Objective To investigate the effects of cadmiun (Cd)on female gonads, especially the stimulating reaction of pituitary and ovary to sexual hormones ; Methods SD female rats were given subcutan eously with the different doses of Cd for 6 weeks(5 days a week, daily) and the estrus cycle of rats was observed, the changes of Cd levels in serum, ovary and uterus were measured, respectively, the gonadotrpic-release hormons (GnRH)stimulating test were carried out in rats, with the serum FSH and LH level determined.30 female mice(KM) were assigned randomly to 3 groups and given the different doses of Cd intraper-itoneally for 5 days (daily) and then the super-ovulation test was perfoned, Results (1)the Cd comtents both in the uterus and the ovary of Cd rats were higher(P<0.05);(2)the ratio of estrus cycle abnormity was higher in the Cd rats than in the control;(3)before the injection of GnRH, there were not significant difference of serum FSH, LH between groups, After the injection of GnRH, the level of serum FSH and LH increased significantly in the Cd rats than in the control(P<0.05);(4)in the super-ovulation test, we had not found the signficant difference of the amount of ova between each groups . Conclusion The ovary may be an important target of Cd effect,causing the gonad toxicity of female rats, such as the abnormity of estrus cycle, Both the lstmulating reaction function and the compensation ability of pituitary to the sexual hormones have been damaged, but no damage was found as regards ovary to super level sexual hormones has not been found.
出处
《卫生毒理学杂志》
CAS
CSCD
北大核心
2002年第1期24-26,共3页
Journal of Health Toxicology
基金
福建省教育厅科研基金资助 (0 0A0 4 8)
