摘要
采用柠檬酸 柠檬酸钠作为缓冲体系 ,使用负高压 ,对Cl-,NO3 -,HCO3 -和H2 PO4 -等 4种常见阴离子进行了分离检测 ,研究了缓冲剂的种类、浓度、pH值及操作电压对分离的影响。在选定的条件下 ,4种离子的定量线性范围 :Cl-5 0× 10 -5mol/L～ 2 5× 10 -3 mol/L ,NO3 -6 0× 10 -5mol/L～ 2 0× 10 -3 mol/L ,HCO3 -5 0× 10 -6mol/L～ 2 0× 10 -4 mol/L ,H2 PO4 -6 0× 10 -5mol/L～ 1 0× 10 -3 mol/L ;检出限 :Cl-1 5× 10 -5mol/L ,NO3 -3 0×10 -5mol/L ,HCO3 -1 0× 10 -6mol/L ,H2 PO4 -2 0× 10 -5mol/L ;峰面积的RSD (n =6 ) :Cl-3 1% ,NO3 -3 3% ,HCO3 -2 6 % ,H2 PO4 -2 9%。并应用选定条件对自来水样品进行了分析。
Capillary electrophoresis with conductivity detection was used to separate and detect chloride (Cl -), nitrate (NO_3 -), bicarbonate (HCO_3 -) and dihydric phosphate (H_2PO_4 -) ions. The proposed method was carried out by using citric acid and sodium citrate as buffer solution, under the condition of a -10 kV high voltage power supply. The effects of various buffers, concentrations, pH values and running voltages on separation were investigated. Under the chosen conditions, the linear ranges of Cl -, NO_3 -, HCO_3 -, and H_2PO_4 - were 5.0×10 -5 mol/L-2.5×10 -3 mol/L, 6.0×10 -5 mol/L-2.0×10 -3 mol/L, 5.0×10 -6 mol/L-2.0×10 -4 mol/L and 6.0×10 -5 mol/L-1.0×10 -3 mol/L respectively. The detection limits were 1.5×10 -5 mol/L, 3.0×10 -5 mol/L, 1.0×10 -6 mol/L, 2.0×10 -5 mol/L and the relative standard deviations (RSD) of migration time were 3.1%, 3.3%, 2.6% and 2.9% respectively. Tap water was analyzed under the same conditions.
出处
《色谱》
CAS
CSCD
北大核心
2002年第2期159-162,共4页
Chinese Journal of Chromatography
基金
SupportedbytheNationalNaturalScienceFoundationofChina(Grant 2 96 75 0 33)andtheNaturalScienceFoundationofGuangdongProvince (Grant 0 0 12 37)
