摘要
目的:建立以毛细管区带电泳、高频电导法测定威灵仙中熊果酸含量的方法。方法:采用未涂层弹性融硅石英毛细管柱(60cm×75μmID,有效长度56cm),以1.2mmol·L-1三乙胺-HCl(pH10.60)、0.24mmol·L-1β-环糊精为运行缓冲液,分离电压为12.5kV,重力进样10s(高度20cm)。结果:熊果酸检测浓度在5.55～111.0mg·mL-1范围内与对照品同内标峰面积的比值呈良好的线性关系(r=0.9993);平均回收率为96.0%,RSD=1.39%(n=6)。结论:本法简便、准确、快速、重现性好,可用于威灵仙中熊果酸的含量测定。
OBJECTIVE：To establish a capillary zone electrophoresis with high frequency conductance method for the determination of ursolic acid in Radix et Rhizoma Clematidis.METHODS： The separation of sample was performed on an uncoated fused silica capillary（60 cm× 75μm ID, effective length： 56 cm） with 1.2 mmol·L^- 1 triethylamine - HCI（pH 10.60） and 0.24 mmol· L^ 1 β- cyclodextrin as running buffer solution.The separation voltage was 12.5 kV and the gravity injection of sample was conducted for 10 s at a height of 20 cm.RESULTS： The calibration curve of ursolic acid was linear over the concentration range of 5.55-111.0 mg · mL^-1（r = 0.999 3） and the average recovery for ursolic acid was 96.0% （RSD = 1.39%, n = 6） . CONCLUSION： The method was proved to be simple, accurate, rapid and reproducible, and suitable for the quantitative determination of ursolic acid in Radix et Rhizoma Clematidis.
出处
《中国药房》
CAS
CSCD
北大核心
2009年第6期436-438,共3页
China Pharmacy
基金
2005年度广东省自然科学基金项目(5002841)
关键词
毛细管电泳法
高频电导
威灵仙
熊果酸
Capillary electrophoresis
High frequency electric conductance
Radix et Rhizoma Clematidis
Ursolic acid
作者简介
讲师。研究方向：中药鉴定、中药质量控制。E-mail：liujzhgd@126．com
通讯作者：教授，硕士。研究方向：中药质量控制。E-mail：fuhaiwu@163．com
