摘要
为了解戊唑醇在黄瓜和土壤中的残留状况及消解动态,建立了戊唑醇在黄瓜和土壤中的高效液相色谱分析方法,并在广东、湖北和陕西3地进行了田间试验.结果表明,采用乙腈提取黄瓜和土壤中的戊唑醇,使用Florisil小柱净化,UVD-HPLC检测,外标法定量,在0.01～1.00 mg/kg添加水平范围内,戊唑醇在黄瓜中的添加回收率为87.32％～95.11％,相对标准偏差为2.79％ ～5.90％;在土壤中平均回收率为86.15％～92.45％,相对标准偏差为2.64％ ～5.17％;黄瓜和土壤中戊唑醇的最小检出量均为2.6× 10-2 ng,最低检测浓度均为0.01 mg/kg.田间试验结果表明,戊唑醇在黄瓜和土壤中的残留消解动态符合方程Ct=C0e-kt,戊唑醇在黄瓜中的半衰期3.16～4.29 d,在土壤中的半衰期4.50～6.09 d.戊唑醇按低剂量112.50 g（ai）/hm^2或高剂量168.75 g（ai）/hm^2施药3次或4次,在最后1次施药2、3d和5d后采收,黄瓜中戊唑醇的残留量均低于1 mg/kg.按试验推荐施药剂量和次数施用戊唑醇,参照我国制订的黄瓜中戊唑醇的最大残留限量标准（1 mg/kg）,所采收的黄瓜是安全的.
In order to know clear residue pollution and degradation trends of tebuconazole in cucumber and soil,an analytical method of HPLC was established for determining tebuconazole residue in cucumber and soil.The field experiments were conducted in Guangdong,Hubei and Shanxi.The result showed that the samples were extracted with acetonitrile,clean-up using a Florisil solid phase extraction cartridge,then determined by reversed phase HPLC equipped with UVD and quantified by external standard.At three spiking levels from 0.01 to 1.0 mg/kg,the recoveries in cucumbers were 87.32 %-95.11%,with RSD 2.79 %-5.90 %,and the recoveries in soil were 86.15 %-92.45 % with RSD 2.64 %-5.17 %.Limit of detection was 2.6 × 10 2 ng,and the lowest concentrations detected were 0.01 mg/kg in cucumber and soil.The degradation of tebuconazole in cucumber and soil could be described with equation：Ct-C0 e-kt,and the half-life of tebuconazole turns was 3.16-4.29 d in cucumber and 4.50-6.09 d in soil.When cucumbers were treated 3-4 times with low 112.50 g（ai）/hm2 or high 168.75 g（ai）/hm2 dosage of tebuconazole,the residues in cucumbers with the last application after 2,3 and 5 d were under 1 mg/kg,which was safe according to the MRL（minimum required petformance level）standard（1 mg/kg）in cucumber from China.
出处
《西南农业学报》
CSCD
北大核心
2014年第3期1159-1164,共6页
Southwest China Journal of Agricultural Sciences
基金
国家农业部农药残留试验研究项目(2012F039)
作者简介
李洪（1989-）,男,湖南临湘人,在读研究生,主要从事农药残留研究,E-mail：lhong8912@163.com
吴忠验（1972-）,男,湖北钟祥人,农艺师,主要从事农业技术推广工作,E-mail：kcld@scau.edu.cn.
