摘要
选取受精后5 d(days post-fertilization,dpf)不同品系斑马鱼,白天和晚上交替给予0.2%高脂饲料8 h和3%葡萄糖溶液16 h,连续4 d,建立糖尿病斑马鱼模型,随机分为模型组、黄地安消胶囊(260 mg·L-1)组、吡格列酮(32 mg·L-1)组,给药组分别水溶暴露给予相应药物,同时设置正常对照组。每组设置30尾斑马鱼,容量为25 mL,28℃培养箱孵育4 d。检测黄地安消胶囊对斑马鱼眼底血管壁厚度、胰岛β细胞荧光强度、巨噬细胞荧光强度、血流速度的改善作用;RT-PCR技术检测血管内皮生长因子(vegfaa)和血管紧张素转化酶(ACE)的表达。结果显示,与模型组比较,黄地安消胶囊能够明显降低血管壁厚度,增加胰岛β细胞和巨噬细胞荧光强度表达,增加体内血流速度,同时降低斑马鱼ACE和vegfaa基因表达,提示黄地安消胶囊可能通过调节ACE和vegfaa基因表达改善斑马鱼血管病变。
Zebrafish of different strains with 5 dpf(5 days post-fertilization) were selected and fed with 0.2% high-fat diet for 8 h and 3% glucose solution for 16 halternatively during the day and night for 4 consecutive days. The zebrafish model was established and randomly divided into model group, Huangdi Anxiao Capsules(260 mg·L-1) group and pioglitazone(32 mg·L-1) group. The drug treatment groups were given the water-soluble drugs, with a volume of 25 mL, and incubated in a 28 ℃ incubator for 4 days. To detect the exposure to the corresponding drugs, the normal control group was set up. Thirty zebrafish were included in each group. The effect of Huangdi Anxiao Capsules on vascular wall thickness, fluorescence intensity of islet beta cells, fluorescence intensity of macrophages, and blood flow velocity of zebrafish were detected. The expressions of vascular endothelial growth factor(vegfaa) and angiotensin converting enzyme(ACE) were detected by RT-PCR. The results showed that compared with the model group, Huangdi Anxiao Capsules can significantly reduce the thickness of the blood vessel wall, increase the fluorescence intensity of islet β cells and macrophages, increase the blood flow velocity in vivo, and decrease the ACE and vegfaa expressions in zebrafish. It is suggested that Huangdi Anxiao Capsules may alleviate zebrafish vascular lesions by regulating the expressions of ACE and vegfaa.
作者
高家荣
郭明飞
方朝晖
姜辉
徐懿乔
单乐天
魏良兵
单莉
GAO Jia-rong;GUO Ming-fei;FANG Zhao-hui;JIANG Hai;XU Yi-qiao;SHAN Le-tian;WEI Liang-bing;SHAN Li(State Adminiztration of Chinese Medicine Preparation Three Laboratories,First Affiliated Hospital of Anhui University of Chinese Medicine,Hefei 230031,China;Hangzhou Hunter Biotechnology lnc ,Hangzhou 310051,China;Zhejiang Chinese Medical University,Hangzhou 310053,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2018年第21期4317-4322,共6页
China Journal of Chinese Materia Medica
基金
国家中医临床研究基地业务建设第二批科研专项(JDZX2015126)
安徽中医药大学青年科学研究项目(2014qn018)
关键词
黄地安消胶囊
糖尿病
血管病变
高糖高脂
斑马鱼
Huangdi Anxiao Capsules
diabetes mellitus
vascular disease
high-sugar and high-fat
zebrafish
作者简介
通信作者:高家荣,主任药师,硕士生导师,研究方向为中药药理学,Tel:(0551)62838556,E-mail:zyfygjr2006@163.com.
