摘要
目的:观察黄地安消胶囊改善HepG2细胞胰岛素抵抗的分子生物学机制。方法:MTT实验分离出黄地安消胶囊含药血清最佳作用浓度和作用时间,免疫荧光技术检测p-IRS-1、PI3K、p-Akt、p-GSK-3β的表达情况;RT-PCR技术检测细胞中IRS-1、PI3K、Akt、GSK-3β的mRNA表达,Western blot技术检测细胞中p-IRS-1、PI3K、p-Akt、p-GSK-3β的相对表达。结果:与正常组相比,模型组中IRS-1、PI3K、Akt表达降低(P<0.05);GSK-3β表达升高(P<0.05);与模型组相比,黄地安消胶囊含药血清组IRS-1、PI3K、Akt升高(P<0.05),GSK-3β表达降低(P<0.05)。结论:黄地安消胶囊可能通过调控IRS-1/PI3K/Akt/GSK-3β信号通路调节蛋白活性,促进糖原合成,降低血糖,改善胰岛素抵抗。
Objective:The aim of this study was to explore the mechanism by Huang Di An Xiao capsules(HDAXC)improves insulin resistance in human hepatocellular liver carcinoma cell line(HepG2).Methods:The optimum concentration and actuation duration were screened from the HDAXC medicated serum by the MTT.The expression of INSR、p-IRS-1、PI3 K、p-Akt and p-GSK-3βwere determined by immunofluorescence technique in each group.Moreover,the mRNA expression of HepG2 cells insulin signaling pathway molecules such as IRS-1,PI3 K and Akt GSK-3βwere investigated by real-time polymerase chain reaction(PCR),and the protein expression of HepG2 cells p-IRS-1、PI3 K、p-Akt and p-GSK-3βwere determined by Western bolt.Results:Compared with the normal group,the expression of IRS-1,PI3 K,Akt were decreased and GSK-3βwas increased in model group(P<0.05).Conversely,after treatment with HDAXC,the expression ofIRS-1,PI3 K,Akt were increased and GSK-3βwas decreased compared with the model group(P<0.05).Conclusions:The possible mechanism of HDAXC may be through IRS-1/PI3 K/Akt/GSK-3βsignaling pathway regulating protein activity,promoting glycogen synthesis,inhibiting gluconeogenesis,reducing blood glucose,improving the insulin resistance,and treatment the T2 DM.
作者
高家荣
郭明飞
单莉
魏良兵
GAO Jiarong;GUO Mingfei;SHAN Li;WEI Liangbing(First Affiliated Hospital of Anhui University of Traditional Chinese Medicine(State Administration of Traditional Chinese Medicine),Hefei 230012,China;Anhui Medical University)
出处
《包头医学院学报》
CAS
2021年第7期48-54,共7页
Journal of Baotou Medical College
基金
国家中医药管理局中医临床研究基地业务建设第二批科研专项课题(JDZX2015126)
