摘要
以黄瓜/酸黄瓜渐渗系‘IL77’为抗病亲本,栽培种‘8419’为感病亲本,通过亲本及F2:6群体筛选,构建了包含137对SSR标记和6对InDel标记的遗传图谱,结合2017年春、秋两季F2:6群体表型统计结果对抗蔓枯病主效QTL进行定位;同时构建极端混池并进行QTL-seq,最终将抗病基因定位到1号染色体24.6～27.1 Mb范围内;通过与参考基因组9930比对发现该区段存在13个基因在外显子区域发生非同义突变,其中Csa1G654870参与RNAi抗性反应,可能与抗蔓枯病过程相关。通过qRT-PCR验证发现,接种前后Csa1G654870在抗病亲本‘IL77’中表达量明显高于感病亲本‘8419’,由此进一步推断Csa1G654870是抗蔓枯病基因。
In this study,a linkage map containing 137 pairs of SSR markers and 6 pairs of InDel markers was constructed using recombinant inbred lines F 2 ︰ ︰ 6 derived from a cross between parental lines ‘IL77’(resistant line from the cross of C. hystrix×C. sativus)and‘8419’(susceptible line). Genetic analysis indicated that the resistance to gummy stem blight(GSB)in‘IL77’was quantitative. Both mapping and BSA-seq analysis were used to detect quantitative trait loci (QTLs)conferring GSB resistance in cucumber leaves. The results showed that the GSB resistance genes were located in the 24.6–27.1 Mb region of chromosome 1. There were 13 candidate genes with exonic nonsynonymous SNP mutation predicted as GSB-resistance related genes in this region. Moreover,bioinformatics analysis and qRT-PCR results provided strong evidence that Csa1G654870 was closely related to GSB resistance process.
作者
张旭
徐建
李季
娄群峰
陈劲枫
ZHANG Xu;XU Jian;LI Ji;LOU Qunfeng;and CHEN Jinfeng(College of Horticulture , State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2018年第11期2141-2152,共12页
Acta Horticulturae Sinica
基金
国家重点研发计划项目(2016YFD0100204-25,2016YFD0101705-5)
国家自然科学基金重点项目(31430075).
关键词
黄瓜
渐渗系
蔓枯病
遗传图谱
混池测序
抗病基因
:cucumber
introgression line
gummy stem blight
linkage map
BSA-seq
resistance gene
作者简介
通信作者:陈劲枫(E-mail:jfchen@njau.edu.cn)
