摘要
以感病×抗病的葡萄种间杂交组合白玉霓×塘尾的F1代 17个单株及塘尾自交一代 16个单株为试材 ,采用BSA法和RAPD技术 ,通过对 15 5个随机引物的筛选 ,获得了一个与中国野生葡萄抗白腐病基因连锁的RAPD标记OPP0 9 76 0 ,并在中国野生葡萄 8个种的 32个株系及欧洲葡萄 16个品种中得到验证。进一步将该DNA片段克隆并测序 ,根据两端序列 ,设计了一对长 2 6bp的特异引物分别扩增供试材料 ,获得了与该RAPD标记相同大小的DNA片段 ,成功地将RAPD标记转化为SCAR标记 。
Bulk segregant analysis(BSA),randomly amplified polymorphic DNA(RAPD)and sequnence characterized amplified region(SCAR)methords were used to tag the white rot resistant genes of grape with 17 individuals of F 1 progeny between Tangwei( Vitis davidii ),white rot resistant and Ugni Blanc( V.vinifera ),White rot sensitive and 16 individuals self pollinated of Tangwei.Among 155 random primers gave distinct band Patterns,One RAPD marker OPP09 760 was tightly linked to a major gene resistant to Coniothyrium diplodiella. Marker OPP09 760 was cloned and sequenced.According to the Sequence,two specific primers were designed to amplify all plant materials.RAPD marker was converted into SCAR marker.One distinct single band only in resistant plants was amplified and its size was the same as that of the RAPD marker.The SCAR marker's popularity was confirmed.And it could be used for the identification of hybrid resistant to Coniothyrium diplodiella.
出处
《园艺学报》
CAS
CSCD
北大核心
2003年第1期6-10,共5页
Acta Horticulturae Sinica
基金
国家自然科学基金资助项目 ( 3 9970 5 2 4)
国家高技术研究发展计划 ( 863计划 )项目 ( 2 0 0 1AA2 41172 )
国家"十五"科技攻关项目 ( 2 0 0 2BA5 15B11)
