摘要
为了筛选新的生防菌种资源,从银杏根、叶中分离出对青枯劳尔氏菌Ralstonia solanacearum有拮抗作用的内生真菌,结合形态特征和ITS序列对该菌株进行了鉴定,分析了菌株发酵液的抑菌特性。结果表明,分离获得的80株内生真菌中有5株对青枯菌有拮抗作用,其中Gbh45具有良好的抑菌效果和遗传稳定性,根据形态特征和ITS序列比对,该菌株被鉴定为球黑孢菌Nigrospora sphaerica。该菌株的发酵产物经紫外照射、不同温度处理后其抑菌活性不发生变化,在碱性条件的耐受能力高于酸性,表明菌株Gbh45的发酵产物在自然条件下稳定性高,该菌对姜青枯病的生物防治具有巨大的应用潜力。
To select new biocontrol agents,several endophytic fungi with antagonism against Ralstonia solanacearum were isolated from Ginkgo biloba roots and leaves.The strains were identified based on morphological characteristics and ITS sequence.Then,the bacteriostatic properties of the fermentation broth of the strain were analyzed.The results indicated that among 80 strains of endophytic fungi,5 strains showed good antagonistic effects against R.solanacearum.Strain Gbh45 showed the strongest antibacterial effect and excellent genetic stability.The strain was identified as Nigrospora sphaerica through morphological characteristics identification and ITS sequence analysis.The fermentation broth of this strain did not change in antibacterial activity after treated with UV irradiation and wide range of temperature,and it also showed stronger tolerance under alkaline conditions than acidic conditions,indicating that fermentation broth of Gbh45 had strong stability under natural conditions. The results implied that Gbh45 had huge potential capacity to be developed into microbial agent to control R. solanacearum.
作者
张祖姣
袁志辉
赵子豪
徐欢
向军
蒲兴锚
何福林
刘小文
ZHANG Zujiao;YUAN Zhihui;ZHAO Zihao;XU Huan;XIANG Jun;PU Xingmao;HE Fulin;LIU Xiaowen(College of Chemistry and Bioengineering,Hunan University of Science and Engineering,Yongzhou 425199,China;Hunan Provincial Engineering Research Center for Ginkgo Biloba,Yongzhou 425199,China;Key Laboratory of Comprehensive Utilization of Advantage Plants Resources in South Hunan,Yongzhou 425199,China)
出处
《中国生物防治学报》
CSCD
北大核心
2018年第6期890-896,共7页
Chinese Journal of Biological Control
基金
国家自然科学基金(31741109
31601696)
国家重点研发计划(2017YFD0201608)
湖南省重点研发计划(2017NK2360)
湖南省自然科学基金(2018JJ2145
2018JJ2146)
湖南科技学院科学研究项目(17XKY002)
湖南省重点实验室开放基金(XNZW15C16)
湖南省大学生研究性学习和创新性实验计划项目(湘教通-2017-205-号)
关键词
银杏
内生真菌
青枯菌
鉴定
抑菌特性
Ginkgo biloba L.
endophytic fungi
Ralstonia solanacearum
identification
antibacterial properties
作者简介
张祖姣,硕士,讲师,E-mail:178624173@qq.com;通信作者:何福林,教授,E-mail:2339695475@qq.com;通信作者:刘小文,博士,副教授,E-mail:lxw1110@126.com。
