摘要
目的 建立双抗体夹心 EL ISA法检测血浆中内毒素 (endotoxin,ET)的方法 ,为临床诊治内毒素血症提供理论依据 .方法 用本实验室自制的两株抗 L PS- m Ab C3 A2 和H3 D3 ,建立检测 ET的双抗体夹心 EL ISA法 ,并与仪器法和鲎试验定性法做比较 ,进行敏感性和准确性实验 .用 3种方法分别测定 40例临床血浆标本 .结果 双抗体夹心法检测ET的最低敏感性为 5 0 ng· L- 1 ,仪器法的敏感性为 1ng·L- 1 ,鲎试验定性法的假阳性率比前 2种方法至少高 17.5 % .3种方法 (双抗体夹心法、仪器法、鲎试验定性法 )对 40份血浆标本检测 ET的阳性率分别为 75 .0 % ,5 7.5 %和 47.5 % .结论 双抗夹心法检测 ET虽敏感性不如仪器法 ,但特异性好 .仪器法的敏感性好 ,但准确性差 ,鲎试验定性法最不准确 。
AIM To establish a double antibody sandwich ELISA method for detecting endotoxin in serum, which can provide theoretic foundation for endotoxemia. METHODS We established the method by using two anti LPS monoclonal antibodies, viz. C3A2 and H3D3. Sensitivity and accuracytests were made and results were compared with instrument detection (ID) and qualitative Limulus test (QLT). 40 clinical samples were detected by three methods respectively. RESULTS The sensitivity of ELISA was 50 ng·L -1 and ID was above 1 ng·L -1 . False positive rate of QLT exceeded the former two methods by at least 17.5%. Positive rates for 40 serum samples of QLT, ID and ELISA were 75%, 57.5% , 47.5%, respectively. CONCLUSION The sensitivity of ELISA is not as good as ID, but its specificity is better. On the contrary, ID's specificity is bad, but its sensitivity is better. QLT is the most inaccurate method and shoud be given up.
出处
《第四军医大学学报》
北大核心
2002年第3期250-253,共4页
Journal of the Fourth Military Medical University
