摘要
为了探讨印60Coγ射线辐照对聚苯乙烯微孔板吸附小分子多肽的影响,建立了检测抗-丙型肝炎病毒 (Hepatitis C virus,HCV)的间接酶联免疫法(Enzyme-linked immunosorbent assay,ELISA)。分别用未经辐 照处理、紫外线(Ultraviolet ray,UV)辐照处理和60Co辐照处理的微孔板吸附重组HCV抗原,再配以辣根 过氧化物酶标记的羊抗人IgG,考察其灵敏度、特异性、均一性和稳定性等相关指标。实验结果表明,经 8 kGy60Co辐照处理的聚苯乙烯微孔板可改善酶联免疫法的测定效果,检测灵敏度和均一性均有所提高。
In order to explore the effect of ^60Coγ-ray irradiation on minor polypeptides absorption of polystyrene microplate, an indirect ELISA detection of anti-HCV was established, ^60Coγ-ray irradiated polystyrene microplates and the controls （without irradiation or UV-irradiated） were applied to absorb recombinant HCV antigens respectively. Cooperated with Bovine antihuman IgG labed HRP, their related indices of sensitivity, specificity, homogeneity and stability were determinated. The results indicated that, optimum dose of the γ-ray irradiation is 8 kGy, and compared with the controls, detection sensitivity and homogeneity of the polystyrene microplate irradiated to 8 kGy could be improved markedly.
出处
《辐射研究与辐射工艺学报》
EI
CAS
CSCD
北大核心
2005年第6期321-324,共4页
Journal of Radiation Research and Radiation Processing
基金
湖南省科技厅重点项目基金(02SSY1001-11)资助
作者简介
冯波，男，1976年8月出生，1998年毕业于武汉大学，生物化学专业，讲师，在读硕士研究生
