摘要
用TTC法测定了刺五加花粉的活力和寿命 ,用联苯胺 -过氧化氢法、开花后套袋法测定了其柱头可授期 ,用扫描电子显微镜观察了刺五加柱头的亚显微形态特征。结果表明 ,刺五加长花丝植株的花粉活力比中花丝植株的高 ;花粉活力及其下降快慢随生境不同而有所差异 ;通常情况下 ,花粉寿命约为 4天。刺五加长花丝植株的柱头一直不具有可授性 ;中花丝植株和短花丝植株的柱头 ,因所处生境不同 ,在开花后 5～ 7、6～ 8或 7～ 9天具可授性 ;生境越郁闭 ,柱头具可授性的时间越晚。具可授性的柱头有独特的形态特征 ,即 :柱头外翻、5或 4裂、湿性。
Pollen viability and life-span of Eleutherococcus senticosus was evaluated by TTC (2,3,5-triphenyl tetrazolium chloride) test, its stigma receptivity was estimated by benzidine-H 2O 2 method and bagging, and the stigma submicroscopic morphology was examined with scanning electron microscopy (SEM). The results as follows: In same habtat, the pollen viability of long-filament (male) E. senticosus was higher than that of middle-filament (bisexual) E. senticosus, the life span of both of them was about 4 days. Moreover, the pollen viability and its decline rate were different according to different plants and different habitats. The stigma of long-filament E. senticosus hadn't receptivity forever, the short-filament (female) and middle-filament (bisexual) E. senticosus had stigma receptivity at 5～7, 6～8, or 7～9 days after blooming according to different habitats, and the more the canopy closure was, the later the stigma receptivity began. When the stigmas began to receive pollens, they were wet, dehiscent and 5 or 4-lobed, and the stigma papillae were kitten's ear-like shape.
出处
《植物研究》
CAS
CSCD
北大核心
2001年第3期375-379,T001,共6页
Bulletin of Botanical Research
基金
黑龙江省自然科学基金资助项目
国家自然科学基金 ( 393915 0 0和 39470 130 )
林业部重点课题 ( 96 -2 8)资助
关键词
刺五加
花粉
花粉活力
柱头
柱头可授性
扫描电子显微镜
Eleutherococcus senticosus
pollen
pollen viability
stigma
stigma receptivity
scanning electron microscopy
