摘要
目的研究人骨保护素（ＯＰＧ）转染小鼠胚胎成肌细胞Ｃ２Ｃ１２后细胞形态及细胞周期的变化。方法采用Ｗｅｓｔｅｒｎ ｂｌｏｔ方法证实ＯＰＧ在真核细胞中表达。对稳定转染ＯＰＧ基因的Ｃ２Ｃ１２细胞的形态、细胞周期等进行检测。结果成功克隆了人ＯＰＧ编码区基因并构建了真核表达载体ｐｃＤＮＡ３－ＯＰＧ。结论人ＯＰＧ编码区基因稳定转染的小鼠胚胎成肌细胞生长状态良好，ＤＮＡ合成旺盛，无诱导细胞凋亡现象，为采用ＯＰＧ基因治疗骨质疏松、骨转移癌等的可行性提供了依据。
Objective To study the effect of human osteopro tegenin(OPG)on cell morphology and cell cycle of C2C12.Method OPG overexpression cell line C2C12was s elected and confirmed by Western blot.The cell morphologic analysis and cell cycle were determined by microscope and flow cytometer.Result The human OPG coding cDNA and it' s mammalian expression vector were obtained.Conclusion Transfection with OPG coding cDNA had no negative effect on target cells,all above suggested that gene therap y using OPG is of feasible methods in the future.
出处
《现代康复》
CSCD
2001年第11期54-55,共2页
Modern Rehabilitation
