摘要
将稀土氧化物加入人胚胎常用体外培养液 Ham'sF-10中,对2细胞小鼠胚胎进行体外培养。发现稀土氧化物浓度为50～100ppm 时,胚胎发育速度及发育率明显高于 Ham'sF-10对照组,且发育加快现象在培养的24小时内己出现,浓度200ppm以上时,促生长效应逐渐减弱;800ppm 以上时,胚胎发育受抑制也在24小时内出现。将在 Ham'sF-10和含50ppm 稀土氧化物的 Ham'sF-10培养液中培养72小时后发育的囊胚分别移植入两组各9只受体母鼠,两组的受体产仔率没有显著性差别,仔鼠未见畸形或生长异常。
This research was conducted to evaluate the effects of rare-earth oxides on embryo development in vitro and embryo transfer.Ham's F-10 media with series concentrations of rare-earth oxides were used for 2-cell mouse embryo culture.The speed and rate of embryo development in a medium containing 50 ppm and 100 ppm rare-earth oxides were higher than those of in F-10 control group,especially within 24 hrs.The dosages of rare-earth oxides below 5 ppm and above 200 ppm had no significant effect on embryo development.Dosage over 800 ppm,inhibited embryo development within 24 hrs in culture.The blastocyst cultured in normal F-10 medium and F-10+50 ppm rare-earth oxides medium were transferred separately to two groups as control and experimental, each group contains 9 mice.Successful rale of embryo transfer between two groups has no significhnt different.There were no malformation and abnormal development.
出处
《暨南大学学报（自然科学与医学版）》
CAS
CSCD
1990年第1期97-103,共7页
Journal of Jinan University(Natural Science & Medicine Edition)
关键词
稀土元素
胚胎培养
胚胎移植
Rare-earth element
Medium
Embryo culture
Embryo transfer
