摘要
通过构建脆壁克鲁维酵母 (Kluyveromycesfragilis)蛋白二硫化物异构酶基因KfPDI强表达单元并克隆到不同类型的酵母载体上转化酵母菌株 ,经筛选得到了带有不同拷贝数KfPDI基因的酵母转化子。酶活性测定结果表明这些转化子中的蛋白二硫化物异构酶表达水平不仅与其KfPDI基因拷贝数有关 ,而且与该基因是否整合入宿主染色体也密切相关。
A KfPDI gene high expression cassette was constructed and cloned into different type of vectors. Some transformants with multi copy number of integrated KfPDI gene were obtained by introducing the expression vectors into yeast Kluyveromyces fragilis. By the examination of protein disulfide isomerase activity, the result showed that the copy number of the KfPDI gene in the yeast transformants was closely related to the level of PDI activities. The K. fragilis strains for high expression of KfPDI gene can be used as genetic engineering host to enhance the secretion efficiency of foreign proteins.
出处
《高技术通讯》
EI
CAS
CSCD
2001年第9期23-27,共5页
Chinese High Technology Letters
基金
863计划资助项目 (863 10 2 11 0 2 0 2 )
