摘要
用各种不同培养基对马铃薯四倍体、双单倍体栽培种及有结薯习性的野生种进行了试管微型薯诱导。在其它条件不变时,用食用白糖替代蔗糖可以获得同样的诱导效果,能大大降低成本。液体培养较固体培养效果好。BAP 的诱导效果随基因型不同而异,但并不一定是必需的。香兰素补加到液体培养基中对提高诱导效果有一定作用。试验表明用 MS 加10%食用白糖或 MS 加10%白糖再补加一定浓度香兰素的液体培养基,在黑暗条件下室温于20～28℃培养是高效率生产试管微型薯较好的方法。
Microtubers were induced from 6 tetraploid cultivars,7 dihaploids and 3 wildspecies in different mediums.The same efficiency could be achieved by usingsugar in place of sucrose without changing any other contents in the medium. This could greatly reduce the cost of microtuber production.The effect of BAPvaried with genotypes of potato germplasm,but it was not essential,Vanillincould improve the effect of microtuber inducing.The result indicated that it wasa better method to produce microtuber by using medium consisting of MS.10%sugar and 50～100 mg/l vanillin in a dark environment at the temperature rangingfrom 20 to 28℃.
出处
《马铃薯杂志》
1991年第4期193-198,208,共7页
Chinese Potato Journal
