摘要
以两个马铃薯普通栽培种“Atlantic”和“甘农薯2号”的无毒试管苗为材料,研究了离体条件下,GA3和IAA对匍匐茎发生和结薯的影响,及在此条件下固体培养、固液双层培养和液体培养3种不同培养方式对马铃薯微型薯诱导和发育的影响.试验结果表明:GA3和IAA在诱导匍匐茎发生和增加单株结薯数上有重要作用,尤以液体培养条件下的效果最为明显;激素诱导下3种培养方式对微型薯的诱导效率在单瓶薯数、单瓶薯重、薯块平均直径及结薯率等方面差异极显著,在单薯重上差异显著,其诱导效应依次为:液体培养>固液双层培养>固体培养.
Virus-free planlets of potato （Solanum tubersum L. ） cultivars‘ Atlantic’ and ‘ Gannongshu No. 2’ were employed in the experiment. The effects of GA3 and IAA on the formation of stolons and produltion of microtubers,and the effects of solid culture, double layer culture and liquid culture on induction and development of potato microtubers under in vitro condition were investigated. The results indicated that GA3 and IAA had significant influence on the stolons formation and producing more microtubers per plantlet,especially in liquid culture. For the number, the weight and the average diameter of microtubers per vessel, there existed significant differences among three culture ways, and it had difference for the weight of single microtuber. The liquid culture was the best to produce the potato microtubers.
出处
《甘肃农业大学学报》
CAS
CSCD
2006年第1期16-19,共4页
Journal of Gansu Agricultural University
基金
国家高技术研究发展计划"863"项目(编号:2001AA241131)
作者简介
彭晓莉（1978-），女，甘肃榆中人，在读硕士研究生．主要从事马铃薯分于育种研究．
通讯作者：王蒂，男，教授，博士生导师，主要从事马铃薯遗传育种研究．
