摘要
目的:考察紫花地丁对巨噬细胞TOLL样受体表达的影响,初步探索该药干预巨噬细胞功能的部分机制。方法:采用紫花地丁水煎剂灌胃干预清洁级SD大鼠常规制备含药血清,按一定浓度于96孔板中与小鼠腹腔巨噬细胞共孵,24 h后中性红法检测巨噬细胞吞噬活性,并采用rt-PCR法检测TLR-1、TLR-2、TLR-3、TLR-4、TLR-5mRNA的表达。结果:与相同浓度正常血清对照组比较(1)紫花地丁含药血清各浓度组巨噬细胞中性红吞噬量明显升高(P<0.01);(2)紫花地丁含药血清一定浓度组巨噬细胞TLR-1、TLR-2和TLR-3 mRNA表达量表现出升高或降低(P<0.05或P<0.01);各浓度组巨噬细胞TLR-4mRNA表达量无显著变化(P>0.05);各浓度组巨噬细胞TLR-5 mRNA表达量显著升高(P<0.01)。结论:紫花地丁含药血清对巨噬细胞吞噬功能具有明显促进作用,其机制可能与该药调控巨噬细胞部分TOLL样受体表达有关。
Objective：To investigate effects of viola on macrophage TOLL-like receptor expression ,and tentatively explore the partial mechanism of viola intervention on macrophage function.Methods： Using viola water decoction lavage intervention in clean grade SD rats of conventional preparation containing serum ,In a certain concentration coculture with mouse peritoneal macrophages in 96-well plates.After 24 hours,cell phagocytosis activity was determined by neutral red uptake assay ,changes in expression levels of TLR-1,TLR-2,TLR-3,TLR-4,TLR-5 mRNAs were determined by RT-PCR.Results：Compared with the same concentration of normal serum group ：（1 ） viola-containing serum group macrophage activity was significantly increased （ P〈0.01 ）;（ 2 ） Within certain concentration,viola containing serum group with increased or decreased expression levels of TLR -1,TLR-2,TLR-3 mRNA（P〈0.05 or P〈0.01 ） ,the expression level of TLR-4 was not significantly altered in each group （ P〉0.05 ） ,and the expression level of TLR-5 was significantly increased in each group （ P〈0.01 ）.Conclusion：Viola-containing serum has obvious promoting effect on macrophage phag-ocytosis,the mechanism of which may be related to that this drug regulates and controls part of macrophage TOLL -like receptor expres-sion.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2014年第6期759-762,共4页
Chinese Journal of Immunology
基金
贵州省委组织部人才办特助项目(TZJF-2009年39号)
贵州省科技厅国际科技合作项目(黔科合外G字[2010]7014号)
贵阳中医学院研究生创新计划项目(ZYYCX12015)
作者简介
作者简介：张智伟（1987年-），男，主要从事细胞免疫学研究，E-mail：248445737@qq．com。
通讯作者及指导教师：田维毅（1972年-），男，教授，主要从事细胞免疫学研究，E-mail：tianweiyi1972@sina．com。
