摘要
采用高效液相与电感耦合等离子体的联用测定干制海产品中的砷形态与含量。选取了阴离子柱Hamiltion PRP X100,以碳酸铵和水为流动相,采用梯度洗脱的方式对6种砷形态(亚砷酸盐、砷酸盐、单甲基砷、双甲基砷、砷甜菜碱及砷胆碱)进行分离。对影响液相的各因素进行了优化,得到6种物质的检出限为0.3～1.0 ng/g,RSD均小于5%。并将此方法成功应用于干制海产品中砷形态的分析检测。
A coupling between high-performance liquid chromatography （HPLC） and inductively coupled plasma-mass spectrometry （ICP-MS） was used for arsenic speciation and quantization in dry seafood products. An anion-exchange column （Hamiltion PRP X100） was found suitable for the simultaneous separation of inorganic and organic species. Six compounds （arsenite, arsenate, monomethylarsonic acid, dimethylarsinic acid, arsenobetaine and arsenocholine） were separated using 50 mmol/L ammonium carbonate solution/H2O solution with a gradient elution. The chromatographic parameters affecting the separation were optimized. The detection limit for six arsenic compounds was from 0.3 ng/g to 1.0 ng/g. The RSD was better than 5% for all investigated compounds. This method was successfully applied in dry seafood in μg/g level.
出处
《食品科技》
CAS
北大核心
2014年第6期303-307,共5页
Food Science and Technology
基金
国家自然科学基金项目(21311037)
作者简介
李祎（1988-），女，硕士研究生，研究方向为食品安全。
通讯作者
