摘要
目的 观察不同剂量乙酰胺对毒鼠强（tetramine,TET）中毒大鼠脑皮层组织病理学改变,为应用乙酰胺治疗TET中毒提供依据.方法 二级SD大鼠80只,随机分为生理盐水对照组、二甲基亚砜水溶液对照组、TET染毒组、2.88 g/kg·d-1乙酰胺治疗组和5.68 g/kg·d-1乙酰胺治疗组,每组16只动物.染毒组和治疗组禁食后给予TET灌胃染毒,染毒后分别肌注生理盐水和不同剂量乙酰胺,连续5d,并分别于治疗处理后3h、12h、48 h和7d取材,取材后通过光学显微镜和电子显微镜观察脑皮层组织病理学改变.结果 （1）光学显微镜观察：TET染毒组主要为脑皮层组织缺氧性改变,随时间延长逐渐加重,给予乙酰胺治疗后缺氧性改变有所减轻,2.88 g/kg·d-1乙酰胺治疗组较5.68 g/kg·d-1乙酰胺治疗组减轻明显.（2）电子显微镜观察：TET染毒后的主要改变为神经元细胞呈凋亡样改变;给予乙酰胺治疗后凋亡样改变的细胞不同程度减少,2.8 g/kg·d-1乙酰胺治疗组较5.6 g/kg·d1乙酰胺治疗组减少明显.结论 给予乙酰胺治疗后,未发现与TET协同作用的中枢毒性作用的脑皮层病理学表现,且给予2.8 g/kg·d-1乙酰胺治疗可能在一定程度减轻中毒后中枢神经损害,而增加乙酰胺治疗剂量,其改善中枢损害的作用有限.
Objective To observe the effect of different doses of acetamide on the histopathology in the cerebral cortex of rats with tetramine （TET） poisoning and to provide a basis for the treatment of fluoroacetamide poisoning with acetamide. Methods Eighty clean Sprague-Dawley rats were randomly divided into five groups： saline control group, dimethyl sulfoxide water solution control group, TET poisoning group, acetamide （2.88 g/kg/d） treatment group, and acetamide （5.68 g/kg/d） treatment group, with 16 rats in each group. Rats in the poisoning group and treatment groups were poisoned with TET by intragastric administration after fasting; then, saline was injected intramuscularly into rats of the poisoning group, and different doses of acetamide were injected intramuscularly into rats of treatment groups; the course of treatment was 5 d. At 3 h, 12 h, 48 h, and 7 d after treatment, the cerebral cortex was harvested from rats in each group, and the histopathologieal changes in the cerebral cortex were evaluated under light and electron microscopes. Results The light microscopy showed that the TET poisoning group had hypoxia changes in the cerebral cortex, which worsened over time; the treatment groups had reduced hypoxia changes, and the acetamide （2.88 g/kg/d） treatment group had more reduction than the acetamide （5.68 g/kg/d） treatment group. The electron microscopy showed that the apoptosis of neuronal cells were the main pathological changes in the TET poisoning group; the treatment groups had reduced apoptotic changes, and the acetamide （2.88 g/kg/d） treatment group had more reduction than the acetamide （5.68 g/kg/d） treatment group. Conclusion No pathological changes associated with the synergistic toxic effect of acetamide and TET are found in the cerebral cortex. Aeetamide （2.88 g/kg/d） could reduce central nervous lesions, but the efficacy is not improved after increasing the dose. For patients who cannot be identified with TET or fiuoroacetamide poisoning, acetamide could be considered for treatment.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2014年第4期289-292,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
关键词
中毒
毒鼠强
乙酰胺
病理
脑皮层
Poisoning
Tetramine
Acetamide
Pathology
Cerebral cortex
