摘要
目的：观察羟基红花黄色素A（HSYA）对激素诱导兔骨髓间充质干细胞（BMSCs）内成骨标志物碱性磷酸酶、Cbfαl及Ⅰ型胶原表达的影响，探讨其防治激素性股骨头缺血性坏死的作用机制。方法：健康成年新西兰大白兔15只，体重0．9～1．3 kg，腹腔注射麻醉后，无菌条件下作胫骨和髂前上棘骨髓腔穿刺抽取骨髓血，分离出BMSCs，体外培养并传代，取第3代生长状态良好的BMSCs随机分为空白组，模型组及羟基红花黄色素A低、中、高剂量组。模型组用大剂量地塞米松诱导BMSCs成脂分化，抑制其成骨分化；羟基红花黄色素A低、中、高剂量组加入地塞米松作用的同时给予羟基红花黄色素A干预；空白组无特殊处理。1周后检测各组细胞内成骨标志物碱性磷酸酶活性、Cbfαl及Ⅰ型胶原mRNA的表达。结果：模型组兔BMSCs内碱性磷酸酶活性较空白组明显下降（P＜0．01），Cbfαl及Ⅰ型胶原mRNA的表达也明显降低（P＜0．01），而HSYA各组较模型组均有明显升高（P＜0．05或P＜0．01）。结论：羟基红花黄色素A治疗激素性股骨头缺血坏死的机制可能与对抗激素诱导下的BMSCs成骨分化减少有关。
Objective：To observe the effect of Hydroxy Safflower Yellow A （HSYA） on the expression of osteogenic markers,such as alkaline phosphatase,Cbfαl and type I collagen,and explore the mechanism of HSYA in the prevention and treatment of glucocorticoid induced ischemic necrosis of femoral head. Methods：Fifteen healthy and adult New Zealand white rabbits were collected and weighted 0.9 to 1.3 kg. The rabbits were injected abdominally with anesthetic drugs ,then received marrow cavity puncture of tibia and anterior superior iliac spine to get bone marrow blood. Rabbits bone marrow mesenchymal stem cells （BMSCs） were separated from the bone marrow blood,cultured in vitro and passaged. The 3rd generation of BMSCs which had good growth condition were randomly divided into blank group ,model group and HSYA groups with different doses. The BMSCs in model group were treated with high dose of dexamethasone to induce adipogenic differentiation of cells cultured in vitro,and inhibit osteogenic differentiation. The BMSCs in HSYA groups received high dose of dexamethasone and different concentrations of HSYA simultaneously. The blank group received not any special handling. After a week ,the expressions of alkaline phosphatase,Cbfαl and type I collagen mRNA were detected. Results：The alkaline phosphatase activity was signifi-cantly decreased in BMSCs of the model group as compared with the blank group （P〈0.01）,and the expression of Cbfαl and type I collagen mRNA were also decreased significantly （P〈0.01）. The alkaline phosphatase activity was significantly in-creased in BMSCs of each HSYA group as compared with the model group （P〈0.05 or P〈0.01）,and the expression of Cbfαl and type I collagen mRNA were also increased significantly （P〈0.05 or P〈0.01）. Conclusion：The mechanism of HSYA may be related to the effect of antagonism to the reduced osteogenic differentiation induced by glucocorticoid.
出处
《中国骨伤》
CAS
2014年第3期224-228,共5页
China Journal of Orthopaedics and Traumatology
基金
国家自然科学基金面上资助项目(编号:465744)~~
关键词
股骨头坏死
羟基红花黄色素A
骨髓间充质干细胞
成骨分化
Femur head necrosis
Hydroxy Safflower Yellow A
Bone marrow mesenchymal stem cells
Osteogenicdifferentiation
作者简介
通讯作者：齐振熙E-mail：zxq@fjtcm．edu．cn
