摘要
为研究多环芳烃对呼吸系统的毒性作用,采用不同浓度的菲体外处理肺泡巨噬细胞(Alveolar macrophage,AM),4 h后测定细胞脂质过氧化水平以及过氧化物酶活性.同时于激光共聚焦显微镜下观察细胞线粒体的融合/分裂状态.研究结果表明:(1)菲使肺泡巨噬细胞谷胱甘肽(Glutathione,GSH)和超氧化物歧化酶(Superoxide dismutase,SOD)含量下降、丙二醛(Malondialdehyde,MDA)含量增加,且呈现剂量-效应关系;(2)菲使得AM线粒体融合/分裂出现异常,细胞内巨型线粒体数目增加.
Polycyclic aromatic hydrocarbons （PAHs） are one group of persistent organic pollutants, and are toxic to plants and other living organisms including human beings. To study the toxic effect of PAHs on respiratory system, we investigated the effects of phenanthrene （Phe） on alveolar macrophages（AM）. AM were treated with phenantrene at different concentrations, and the level of lipid peroxidation （LPO） , and the activities of antioxidant enzymes were determined after 4 h. At the same time mitochondria fusion and fission of AM were observed under confoeal scanning laser microscopy （CSLM）. The results showed： （1） phenanthrene caused the decrease of AM GSH and SOD activities and increased MDA content in a dose-response manner; （2） phenanthrene led to abnormal fusion and fission of mitochondria, and the number of megamitoehondrion in cells increased.
出处
《环境化学》
CAS
CSCD
北大核心
2014年第4期551-555,共5页
Environmental Chemistry
基金
国家自然科学基金(21177078)
山西省自然科学基金(2011011009-2)资助
关键词
菲
肺泡巨噬细胞
毒性作用
phenanthrene, alveolar macrophage, toxicity.
作者简介
通讯作者，E—mail：weihaiying@sxu．edu．cn
